Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa

Table 1

Primer sequences used for , , , , and genes detection.

Target gene	Primer sequences (5′-3′)	Temp in °C	Target size bp
CTX-M forward primer	ATG TGC AGY ACC AGT AAR GTK ATG GC	58.7	336 bp
CTX-M reverse primer	ATC ACK CGG RTC GCC NGG RAT	59.3
CTX-M1 probe	FAM-CCC gAC AgC Tgg gAg ACg AAA CgT	70.2
CTX-M2 probe (gr 2)	YAK-CAggTgCTTATCgCTCTCgCTCTgTT	66.3
CTX-M9 all/1 probe	LC640-Cg AC AAT ACNgCC ATg AA	41.0
CTX-M9 probe	LC610-CTggATCgCACTgAACCTACgCTgA	53.0
TEM forward primer	AAG TTC TGC TAT GTG CGG TA	59.7	180 bp
TEM reverse primer	TGT TAT CAC TCA TGG TTA TGG CAG C	59.5
SHV forward primer	CAG GAT CTG GTG GAC TAC T	57.2	195 bp
SHV reverse primer	GTC AAG GCG GGT GAC GTT	59.3
SHV-A primer	AAG GCG GGT GAC GTT GTC	59.3
SHV-S primer	CCG GTC AGC GAA AAA CAC	57.0
SHV probe	Cy5-TCT GGC GCA AAA AGG CAG TCA-BBQ	64.5
NDM forward primer	GAC CGC CCA GAT CCT CAA	57.5	51 bp
NDM reverse primer	CGC GAC CGG CAG GTT	60.2
NDM probe	FAM-TGG ATC AAG CA+GGA+GAT-BBQ	48.8
KPC forward primer	GGC CGC CGT GCA ATA C	58,6	61 bp
KPC reverse primer	GCC CAA CTC CTT CA	59,6
KPC probe	Cy5-TGA TAA CGC GCG CAA TTT GT-BBQ	68.9

Relevant positive strains for Klebsiella used as positive control in rPCR: CTX-M (group 1)—E. coli ATCC 25922, CTX-M (all groups except group 1)—CAP2006-D17, TEM—K. pneumoniae ATCC 51503, SHV—K. pneumoniae ATCC 700603, KPC—K. pneumoniae ATCC BAA 1705, NDM—K. pneumoniae ATCC BAA 21246. Obtained from NICD (National Institute of Communicable Diseases, Johannesburg, South Africa).