Differential Microbial Diversity in Drosophila melanogaster: Are Fruit Flies Potential Vectors of Opportunistic Pathogens?
Table 1
Identification and distribution of fungi and bacteria on and in fruit flies. GenBank accession numbers are for ITS sequences of fungi and 16S rDNA sequences for bacteria. The last six columns show numbers of each microorganism isolated with the following protocols: flies that walked across petri plates; flies washed in 0.01% Tween 80; surface-sterilized flies; and guts removed from flies.
Strain
Species
GenBank accession ID
Highest hit
GenBank accession ID
% identity
External isolation
Internal isolation
Total
value
Walked
Washed
Insect
Gut
Fungus
H137
Candida inconspicua
KU238836
Candida inconspicua
KT207004
100
27
20
23
14
84
0.275
H35
Penicillium crustosum/commune
KU238837
Penicillium commune
KR012904
100
10
5
6
7
28
0.705
H127
Issatchenkia hanoiensis
KU238838
Issatchenkia hanoiensis
FJ153178
99
1
1
9
2
13
0.013
H66
Aspergillus versicolor/sydowii/nidulans
KU238839
Aspergillus sydowii
KT989398
100
3
1
0
4
8
1.000
H11
Aspergillus fumigatus
KU238840
Aspergillus fumigatus
AB298709
100
2
1
2
0
5
0.655
H37
Fusarium sp.
KU238841
Fusarium equiseti
HQ332532
100
1
1
2
1
5
0.655
H44
Galactomyces candidum/geotrichium
KU238842
Galactomyces candidum
KJ579946
99
1
1
1
1
4
1.000
H102
Pichia membranifaciens
KU238843
Pichia membranifaciens
FJ231462
99
3
0
1
0
4
0.317
H7
Rhizopus sp.
—
—
—
—
0
3
0
0
3
0.083
H24
Fusarium equiseti/oxysporum
KU238844
Fusarium equiseti
KJ174399
100
2
0
0
1
3
0.564
H77
Penicillium citrinum/griseofulvum
KU238845
Penicillium citrinum
KU681430
98
0
2
1
0
3
0.564
H31
Aspergillus niger
KU238846
Aspergillus niger
KP748369
99
1
1
1
0
3
0.564
H46
Geotrichum candidum
KU238847
Geotrichum candidum
KF713518
100
1
1
0
0
2
0.157
H69
Aspergillus flavus/oryzae
KU238848
Aspergillus flavus
KU360621
100
0
1
0
0
1
0.317
H70
Aspergillus niger/tubingensis
KU238849
Aspergillus niger
EU645723
98
0
0
1
0
1
0.317
Bacteria
B82
Klebsiella sp.
KU238850
Klebsiella sp.
FN178363
99
5
6
8
13
32
0.077
B5
Bacillus sp.
KU238851
Bacillus pumilus
KU517819
99
7
5
4
6
22
0.670
B30
Klebsiella oxytoca
KU238852
Klebsiella michiganensis
KP717391
99
6
6
3
5
20
0.371
B44
Klebsiella pneumoniae/variicola
KU238853
Klebsiella variicola
KT895843
99
3
1
4
6
14
0.109
B22
Erwinia sp.
KU238854
Uncultured Erwinia sp.
HE575588
99
3
4
3
1
11
0.366
B105
Bacillus pumilus/safensis
KU238855
Bacillus pumilus
KU239978
99
2
0
3
4
9
0.096
B39
Stenotrophomonas maltophilia
KU238856
Stenotrophomonas maltophilia
LT222226
99
2
2
3
2
9
0.739
B84
Micrococcus luteus/yunnanensis
KU238857
Micrococcus luteus
KT901825
100
4
0
4
0
8
1.000
B43
Alcaligenes faecalis
KU238858
Alcaligenes faecalis
KU179370
99
2
1
1
2
6
1.000
B8
Microbacterium oxydans
KU238859
Microbacterium oxydans
KT580637
99
0
1
0
0
1
0.317
Drosophila
D1
Drosophila melanogaster
KU238860
Drosophila melanogaster
KP161877
99
D2
Drosophila melanogaster
KU238861
Drosophila melanogaster
KP161877
99
D3
Drosophila melanogaster
KU238862
Drosophila melanogaster
KP161877
99
Asterisk represents significant differences by Chi square test.
