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International Journal of Microbiology
Volume 2017, Article ID 9042125, 4 pages
https://doi.org/10.1155/2017/9042125
Research Article

Baseline Antibody Titre against Salmonella enterica in Healthy Population of Mumbai, Maharashtra, India

1Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai, Maharashtra, India
2Shri Bhausaheb Hire Government Medical College and Hospital, Dhule, India
3Department of Pathology, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai, Maharashtra, India

Correspondence should be addressed to Rucha Patki; moc.liamg@iktapaahcur

Received 10 May 2017; Accepted 3 July 2017; Published 31 August 2017

Academic Editor: Barbara H. Iglewski

Copyright © 2017 Rucha Patki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. Method. Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. Results. Out of 400 individuals providing samples, 78 (19.5%) individuals showed antibody titres ≥ 1 : 40 for at least one antigen and 322 (80.5%) showed no agglutination. The baseline antibody titres against O antigen and H antigen of Salmonella enterica serotype Typhi were found to be 1 : 40 and 1 : 80, respectively. Similarly, the baseline antibody titres for the H antigen of Salmonella enterica serotypes Paratyphi A and Paratyphi B were found to be 1 : 40 and 1 : 80, respectively. Conclusion. Thus, it was noted that the diagnostically significant cutoff of antibody titre from acute phase sample was ≥ 1 : 80 for S. Typhi O antigen and titre of ≥ 1 : 160 for both S. Typhi H antigen and S. Paratyphi BH antigen. Antibody titre of ≥ 1 : 80 can be considered significant for S. Paratyphi AH antigen.