Research Article

Enterococcus faecium TIR-Domain Genes Are Part of a Gene Cluster Which Promotes Bacterial Survival in Blood

Figure 6

Detection of TirEs and Hp1 in the bacterial cell lysate, supernatant, and MV. (a–c) TirE1 (18.5 kDa), Hp1 (55 kDa), and TirE2 (33 kDa) were immunoprecipitated from the lysed pellet (P) or bacterial supernatant (S) after 4 h or from overnight culture (on) and detected in western blot with anti-TirE1, anti-Hp1, or anti-TirE2 serum. Shown are samples from the E1162 and K60-39, purified proteins (TirE1 with his tag 20.4 kDa (lane 5 in (a)), Hp1 with his tag 57 kDa (lane 5 in (b)), and TirE2 with his tag 35 kDa (lane 5 in (c))), and Magic marker (M) with size indicated in kDa. (d) EmPAI values indicating the protein abundance of TirE1, Hp1, and TirE2 proteins in membrane vesicles derived from K60-39 and DO.