Research Article | Open Access
Maria L. Schmitz Fontes, Heloísa Fernandes, Manoela Brandão, Mariana Coutinho Hennemann, Raquel Aparecida Loss, Valdelúcia Maria Alves de Souza Grinevicius, Denise Tonetta, Karina Cesca, Mônica Hessel Silveira, Mara Bedin, Derce Recouvreux, Regina Vasconcellos Antônio, "Bacterioplankton Activity in a Meso-eutrophic Subtropical Coastal Lagoon", International Journal of Microbiology, vol. 2018, Article ID 3209605, 8 pages, 2018. https://doi.org/10.1155/2018/3209605
Bacterioplankton Activity in a Meso-eutrophic Subtropical Coastal Lagoon
The aim of this study was to investigate whether the bacterioplankton activity in the meso-eutrophic Conceição Lagoon would increase significantly under allochthonous inputs of inorganic nutrients and organic carbon. Abundance and biomass of bacterioplankton were evaluated under three treatments: light (14 h light/10 h dark), complete darkness (dark-control), and nutrient (C + N + P—dark, 100 : 10 : 1) enrichments during 72 h. Nutrient enrichments promoted a significant increase in abundance (maximum of 19.0 ×109 cells·L−1 in the first 32 hours) and biomass of the heterotrophic bacterioplankton, which induced the formation of large clusters. Bacterial biomass remained constant in the non-enriched incubations (dark-control and light). Bacterial growth rates were significantly higher after nutrient additions (1.35 d−1), followed by control (0.79 d−1), and light (0.63 d−1) treatments, which were statistically equal (). Bacterial production rates were also significantly higher under nutrient additions (1.28 d−1), compared to the control and light (0.50 d−1 and 0.44 d−1, respectively), demonstrating that bacterial growth and production in this meso-eutrophic lagoon are under an immediate “bottom-up” regulation, followed by a potential top-down effect. These facts reinforce the urgency on improving the local wastewater management plan in order to prevent further expansion of anoxic waters.
The comprehension of the pelagic bacterioplankton abundance and activity in coastal aquatic environments is of major importance in order to predict shifts in the microbial communities in response to frequent changes in such systems . The inputs of organic material and inorganic nutrients to coastal lagoons mainly from watershed runoffs, infiltration, precipitation, air and sea exchanges can have a direct influence on activity and composition of microbial communities and, consequently, in the ecosystem function [2, 3]. Coastal lagoons are located in the transitional region between terrestrial and marine environments, and are considered highly dynamic environments, subjected to abrupt changes in physicochemical variables, residence times, and recipients of multiple sources of nutrients [3, 4].
Heterotrophic bacteria play important roles in the remineralization and transformation processes between dissolved organic carbon (DOC) and particulate organic carbon (POC) [5, 6], which will then be available to higher trophic levels through the microbial loop . The functionality of bacterioplankton, predominantly net sink or link of carbon in a system, will depend on the chemical composition of the DOC pool , if predation is absent or minimized.
However, the cause-effect relationship of labile carbon forms and/or inorganic nutrients on the bacterial activity is not clear yet. Some studies have shown a positive effect of dissolved inorganic nitrogen and phosphate enrichment on marine bacterial growth [9–12], while others demonstrated no effect . With the simultaneous addition of inorganic nutrients and glucose, several studies have shown a significant response in bacterial abundance and production [14–17]. The heterogeneity observed in the results has been related to the different trophic states of each environment .
In coastal ecosystems such as the Conceição Lagoon, a meso-eutrophic coastal lagoon , additional inputs of allochthonous material can stimulate heterotrophic microorganisms and consequently lead to oxygen depletion [19–21]. Anoxia occurrence has increased significantly in the last 15 years in the central area of the lagoon , and its trophic state has changed from oligo-mesotrophic to meso-eutrophic in the same period of time . Thus, we hypothesized that bacterioplankton activity, determined here by bacterial production and growth, would increase in this meso-eutrophic system if exposed to simultaneous external inorganic nutrients' sources and organic matter.
2. Materials and Methods
2.1. Water Sampling and Study Site
A 20 L water sample was collected at subsurface in the central sector of Conceição Lagoon on the 2nd of November, 2011, and was kept at low temperature until transported to the laboratory. The lagoon is located between 27°30′17″ and 27°37′36″S and 48°25′30″ and 48°29′54″W (Figure 1).
Conceição Lagoon is connected to the Atlantic Ocean by a 2 km long channel, which drives a salinity variability in the central sector from 17.6 up to 30 [19, 22]. Water temperature during the experiment was maintained 24°C; usual dissolved inorganic nitrogen (ammonium + nitrate + nitrite) and dissolved inorganic phosphorus (phosphate) concentrations range between 1.0 and 13.2 µM and between 0.09 and 0.52 µM, respectively; with average DIN : DIP ratios above 25 : 1 . Chlorophyll a concentrations average between 3.5 and 4.0 µg·L−1 [19, 20, 22]. Additionally, continuous runoffs rich in untreated (raw) domestic sewage flow directly into its water body [22, 23]. The average domestic sewage volume discharged into the system has been calculated to be around 160 m3 of sewage month−1, corresponding to 300 kg (biological oxygen demand, BOD5 day)−1 . Due to this elevated sewage input, a considerable increase in dissolved inorganic nitrogen (ammonium, nitrite, and nitrate) concentrations has been observed in the lagoon water since 1980s .
2.2. Culture Preparation and Experimental Procedure
Water aliquots were collected from Conceição Lagoon and carefully filtered through a cellulose acetate membrane (Millipore 47 mm in diameter and 0.2 µm in pore size) in the laboratory. One liter of the filtered water or medium (dilution of the sample resulted in a reduction in the amount of grazers ) was transferred onto each of three sterile glass recipients resulting in 70% of filtered medium and 30% of inoculum (whole water—no previous filtration), equivalent to a volume of 1.43 L per replicate. Bacterioplankton cultures received the following labels according to the treatment: (1) light (in situ light intensity and photoperiod: 14 h light, 10 h dark, room temperature); (2) control (dark, room temperature); and (3) nutrients (C + N + P = glucose, NH4Cl, and K2HPO4 additions at final concentrations of 100 µM C : 10 µM N : 1 µM P, dark, room temperature). All treatments were kept under the described conditions for 72 h. The reason why control and nutrients’ treatments were kept in the dark was to prevent the accumulation of autotrophic biomass (presence of photoautotrophic bacteria and picoeukaryotes) throughout the experiment, which would interfere with the results by competition or release of extracellular DOC. On the other hand, light treatment was important for the effects of light/dark cycles on bacterial activity when compared to complete darkness.
2.3. Bacterial Counts and Biomass
Aliquots of 15 ml were taken from each treatment at 12 h intervals and fixed with PFA (paraformaldehyde) at 2% (final concentration), making 7 estimates for each treatment (t0, t12, t24, t36, t48, t60, and t72). The aliquots were used to determine bacterial abundance, biomass, and size or biovolume. One ml of aliquots from each treatment was filtered through 0.2 µm dark polycarbonate membranes (diameter of 25 mm) (Millipore) and stained with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min (1 µg·ml−1, final concentration).
Filters were mounted onto slides, and bacterial cells were counted using an epifluorescence microscope (Olympus BX-40) . From each filter, ten random fields were counted and at least 200 bacterial cells were measured. Length, width, elongation, and area of each cell were measured using the freeware “UTHSCSA Image Tool” (University of Texas Health Science Center, San Antonio, Texas, US) . Bacterial biovolume was calculated from length and width measurements and biomass as function of biovolume using the following algorithm: B = 120 V0.72, where B = biomass; V = biovolume (μm3); 120 = conversion factor in fg·C·μm−3 . Total biomass was calculated by multiplying the average cellular biomass by bacterial abundance.
2.4. Data Analysis
Bacterial growth was calculated as the increase in cellular density from the beginning to the end of the exponential growth period. Bacterial production was calculated in the same manner using bacterial biomass data by the time. Bacterioplankton specific growth rates (µ) and biomass production rates were calculated as the slope of the linear regression of ln-transformed bacterial abundance or biomass with time during the exponential growth . Daily growth rate (daily µ) and daily bacterial production rates were obtained by multiplying the hourly growth rate by 24. Doubling time was calculated by dividing 0.69 (ln 2) by the daily µ. Comparison between bacterial growth rates and production rates (linear regression angular coefficient) was made by analysis of covariance (ANCOVA), with subsequent multiple comparisons performed using Tukey’s test with a statistical significance level of . Density, biomass, and biovolume means were compared by analysis of variance (ANOVA) or t-test, if the case, of (log + 1)-transformed data, followed by Tukey’s test (significance level of ) [30, 31]. Statistical tests were conducted using Statistica 7 software.
Samples showed different abundance and biomass values among treatments and time. The mean number of cells (cells·L−1) in each treatment after 72 h of incubation was higher in the nutrient treatment (7.44 × 109 ± 2.55 × 109 cells·L−1), followed by the dark-control (3.00 × 109 ± 8.04 × 108 cells·L−1) and light flasks (1.77 × 109 ± 2.08 × 108 cells·L−1). Bacterial abundance increased exponentially until 36 h (maximum 1.9 × 1010 cells·L−1), followed by a decrease and a second small increase between 60 and 72 h in the nutrient treatment. In the dark-control treatment, the number of cells increased until 48 h (maximum 7.18 × 109 cells·L−1). In the light flasks, samples showed a lower abundance when compared to the other treatments and maintained approximately constant values throughout the experiment (Figure 2).
Considering cells arrangement in each treatment, there was an increase in the occurrence of cells clustering and size after 36 h of incubation only in the nutrient enrichment, with up to 200 cells clustered together (Figure 3). In addition, the abundance of filamentous bacteria increased between 12 and 24 h in the nutrients treatment, while in the dark-control, a smaller and delayed increase occurred between 36 and 48 h. In the light treatment, constant numbers of filamentous bacteria were observed throughout the experiment (Figure 4). However, no significant differences were observed concerning filamentous bacterial abundance among treatments (one-way ANOVA, ). Considering the relative abundance of filamentous bacteria, a stronger contribution of this portion of the community was detected in the light and dark-control flasks (5.6% and 7.1%, respectively), against 2.5% contribution in the ones that received nutrients.
The bacterial size estimated as cellular biovolume (µm3) has been used to calculate cellular biomass in function of C availability (µg·C·cell−1). As a result, mean cellular biovolume ranged between 6.45 and 13.65 µm3, and biomass, as a consequence, between 417.82 and 678.36 µg·C·cell−1. The highest averages of biovolume were observed in the nutrients treatment at 36 h (13.65 µm3) and in the light at 24 h (13.58 µm3) (Figure 5). High oscillations in the mean biovolume can be attributed to different contributions of filamentous bacteria and to the presence of different bacteria morphotypes. However, biovolume was not significantly different among treatments (one-way ANOVA, ) (Table 1). On the other hand, considering total biomass (carbon per volume unit (mg·C·L−1)), the highest biomass was registered in the nutrients treatment, especially between 24 and 36 h (with a peak of 12.3 mg·C·L−1) (Figure 6), similarly to what was shown for bacterial abundance (Figure 2).
DF = degrees of freedom.
Bacterial abundance and biomass were higher under nutrients additions when compared to the dark-control and light treatments (one-way ANOVA, ) (Table 1). Bacterial growth rates were 0.79 d−1, 1.34 d−1, and 0.63 d−1 in the dark-control, nutrients, and light treatments, respectively. Bacterial production rates oscillated from 0.44 to 1.13 d−1 (Table 2). The ANCOVA result showed that bacterial growth and biomass production rates differed among treatments (ANCOVA: F0.05;(1);2;9 = 9.00 for growth and ANCOVA: F0.05;(1);2;9 = 5.08 for bacterial production). The difference among slopes was tested, and it was observed that bacterial growth and production were significantly higher in the nutrients treatment, while growth and production were similar in light and dark-control incubations (Table 2).
Superscript letters indicate the result of ANCOVA and Tukey test for difference among slopes-q 0.05.
Doubling times (0.51 d−1, 0.87 d−1, and 1.10 d−1 for nutrients, dark-control, and light, respectively) showed that the heterotrophic bacterial community went through two duplications per day in the nutrients treatment, while in natural conditions (dark-control treatment), bacterioplankton duplicated once a day (Table 2).
When changes in cellular biovolume and carbon biomass are considered, slightly different results were observed, since both nutrients and light treatments showed small increases in the parameters at the end of the 72 h incubation period.
Bacterioplankton activity in this meso-eutrophic lagoon was significantly stimulated after the addition of dissolved organic carbon and inorganic nutrients, demonstrating the vulnerability of this system to external sources of organic and inorganic matter.
The average cellular size, 13 µm3, did not vary significantly throughout the experiment; however, bacterial cells showed a great morphological variability. Naturally, isolated marine bacteria show low growth rates under environmental conditions . These authors reported that cells with a biovolume of 0.008 µm3 have growth rates of 0.3 d−1, while 0.3 µm3 cells show growth rates of 1.4 d−1. The average biovolume found in our study was 13 µm3, two orders of magnitude higher than usually found in marine environments. However, it is important to highlight the presence and the relative abundance of filamentous bacteria, especially around 48 h in the control treatment. The predominance of heterotrophic filamentous bacteria has already been described previously in the Conceição Lagoon as positively related to the coccoid cyanobacteria of genus Synechococcus , as shown in Figures 4(b) and 4(c). This has been described as a strategy to avoid predation, when bacteria alter their size towards filamentous forms when exposed to grazing pressure [33–35].
Some studies have suggested that viral lysis and predation by protists are the main regulators of bacterial growth and production, many times more important than resources availability [36–38]. However, in environments with N : P ratios above the Redfield ratio, where phosphorous is the limiting resource, as in Conceição Lagoon [22, 23], bottom-up control of bacterial growth and/or biomass has a much more important role than top-down control by bacterivory [39, 40]. Therefore, grazing cannot be ignored as a secondary regulator on the activity of bacterioplankton. The significant decrease of bacteria abundance between 36 and 60 h in the nutrients treatment was concurrent with a slight decrease in the average bacterial biovolume, which is illustrated by the formation of several clusters of bacterial cells resulting on smaller biovolume when attached to each other. There are a few hypotheses for this outcome: predation scape strategy, increase proximity, and signaling among cells to overcome resource limitation.
In Conceição Lagoon, bacterial growth rates and production doubled after the addition of nutrients, compared to the dark-control and light treatments. In a study carried out with samples from the oligotrophic Sargasso Sea, the additions of nitrogen, phosphorus, and carbon resulted in increasing bacterial production by 7 to 15 times when compared to control or inorganic nutrient additions alone . This finding reinforces that bacterioplankton of oligotrophic environments (under constant starvation and competition pressure with phytoplankton) responds much faster after simultaneous additions of inorganic nutrients and carbon than those of meso-eutrophic environments.
Previous studies have shown contradicting results on the effect of nutrients and carbon enrichment on bacterial growth and secondary production (e.g., [40–46]). For example, a significant increase on bacterial production after the addition of nutrients and carbon are reported in a fjord in Norway , whereas no significant changes were described in the oligo-mesotrophic Uchiumi Bay, Japan . The best explanation for such heterogeneity in growth and productivity of aquatic bacteria has been described to be the ecosystem’s trophic state, as the primary response of bacteria to eutrophication is to increase their specific growth rates . The ecosystem’s trophic state is related to variables that directly or indirectly correlate with the system’s primary production: total organic matter, dissolved oxygen concentration, total phosphorus, light penetration, and phytoplankton biomass indicated by chlorophyll a concentration [49, 50]. As Conceição Lagoon is under eutrophication, with persistent anoxic waters in the bottom of the central sector, the increase in aerobic respiration by microbes can be a major problem for the water quality of this ecosystem.
Thus, increasing nutrient loads (N and P) directly or indirectly (trophic state) will alter bacterial community composition by favouring certain bacterial populations that have the ability to rapidly consume these new available resources [51–55]. Our results show that bacterial activity remains unaltered when exposed to complete darkness (t-test - light x control - ), and that under additions of inorganic nutrients and organic carbon, the total bacterial activity doubles in the first 24 hours. Apparently, there is an indicative of strong competition for nutrients in Conceição Lagoon.
The present study shows similar activities for total (auto + heterotrophic) bacterioplankton and strict heterotrophic bacteria when neither inorganic nutrient nor carbon is added onto the lagoon. Nevertheless, bacterioplankton growth and production were stimulated by carbon, nitrogen, and phosphorous additions, indicating that bacterioplankton biomass in this meso-eutrophic coastal lagoon is likely to be primarily bottom-up regulated. This study suggests that allochthonous carbon and inorganic nutrients via untreated sewage inputs or rainstorm runoffs into the lagoon have the potential to promote a quick response of bacterioplankton and, thus, increase the area and persistency of the “dead zones” within the lagoon. Our results reinforce the importance of an effective waste management plan for the area.
The data used to support the findings of this study are available from the corresponding author upon request.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
- B. Divya, S. O. Fernandes, G. Sheelu, N. Shanta, P. A. L. Bharathi, and D. Chandramohan, “Limno-tolerant bacteria govern nitrate concentration in Mandovi Estuary, India,” Estuarine, Coastal and Shelf Science, vol. 82, no. 1, pp. 29–34, 2009.
- J. J. Barrera-Alba, S. M. F. Gianesella, G. A. O. Moser, and F. M. P. S. Correa, “Influence of allochthonous organic matter on bacterioplankton biomass and activity in a eutrophic, sub-tropical estuary,” Estuarine, Coastal and Shelf Science, vol. 82, no. 1, pp. 84–94, 2009.
- F. A. Esteves, A. Caliman, J. M. Santangelo, R. D. Guariento, V. F. Farjalla, and R. L. Bozelli, “Neotropical coastal lagoons: an appraisal of their biodiversity, functionings, threats and conservation management,” Brazilian Journal of Biology, vol. 68, no. 4S, pp. 967–981, 2008.
- B. Kjerfve, Coastal Lagoon Processes, Elsevier Science B.V., Amsterdam, Netherlands, 1994.
- D. Sigge, Freshwater Microbiology: Biodiversity and Dynamic Interactions of Microorganisms in the Aquatic Environment, vol. 1, John Wiley & Sons Ltd., Chichester, UK, 2005.
- R. G. Wetzel, Limnology, Saunders Publishing, Philadelphia, PA, USA, 1982.
- F. Azam, T. Fenchel, J. G. Field, J. S. Gray, L. A. Meyer-Reil, and F. Thingstad, “The ecological role of water-column microbes in the sea,” Marine Ecology Progress Series, vol. 10, pp. 257–263, 1983.
- M. Warkentin, H. M. Freese, and R. Schumann, “Bacterial activity and bacterioplankton diversity in the eutrophic river Warnow-direct measurement of bacteria l growth efficiency and its effect on carbon utilization,” Microbial Ecology, vol. 61, no. 1, pp. 190–200, 2011.
- D. A. Caron, E. L. Lim, R. W. Sanders, M. R. Dennett, and U. G. Berninger, “Responses of bacterioplankton and phytoplankton to organic carbon and inorganic nutrient additions in contrasting oceanic ecosystems,” Aquatic Microbial Ecology, vol. 22, pp. 175–184, 2000.
- M. P. Hoch and D. A. Bronk, “Bacterioplankton nutrient metabolism in the Eastern Tropical North Pacific,” Journal of Experimental Marine Biology and Ecology, vol. 349, no. 2, pp. 390–404, 2007.
- L. R. Pomeroy and D. Deibel, “Temperature regulation of bacterial activity during the spring bloom in newfoundland coastal waters,” Science, vol. 233, no. 4761, pp. 359–361, 1986.
- M. M. Sala, F. Peters, J. M. Gasol, C. Pedrós-Alió, C. Marrase, and D. Vaque, “Seasonal and spatial variations in the nutrient limitation of bacterioplankton growth in the northwestern Mediterranean,” Aquatic Microbial Ecology, vol. 27, pp. 47–56, 2002.
- D. Ichinotsuka, T. Katan, H. Takeoka, and S. Nakano, “Effects of nutrient supplies on the growth rates of planktonic bacteria in Uchiumi Bay, Japan,” Aquatic Biology, vol. 9, pp. 123–130, 2010.
- C. A. Carlson, S. J. Giovannoni, D. A. Hansel et al., “Effect of nutrient amendments on bacterioplankton production, community structure, and DOC utilization in the northwestern Sargasso Sea,” Aquatic Microbial Ecology, vol. 30, pp. 19–36, 2002.
- M. J. Church, D. A. Hutchins, and H. W. Ducklow, “Limitation of bacterial growth by dissolved organic matter and iron in the Southern Ocean,” Applied and Environmental Microbiology, vol. 66, no. 2, pp. 455–466, 2000.
- S. P. Donachie, R. James, J. R. Christian, and D. M. Karl, “Nutrient regulation of bacterial production and ectoenzyme activities in the subtropical North Pacific Ocean,” Deep Sea Research Part II: Topical Studies in Oceanography, vol. 48, no. 8-9, pp. 1719–1732, 2001.
- L. R. Pomeroy, J. E. Sheldon, W. M. Sheldon Jr., and F. Peters, “Limits to growth and respiration of bacterioplankton in the Gulf of Mexico,” Marine Ecology Progress Series, vol. 117, pp. 259–268, 1995.
- V. E. C. Silva, D. Franco, A. L. Fonseca, M. L. Schmitz Fontes, and A. R. Donnangelo, “Space time evolution of the trophic state of a subtropical lagoon: Lagoa da Conceição, Florianópolis Island of Santa Catarina, Brazil,” RBRH, vol. 22, p. e10, 2017.
- M. L. S. Fontes and P. A. Abreu, “Spatiotemporal variation of bacterial assemblages in a shallow subtropical coastal lagoon in Southern Brazil,” Microbial Ecology, vol. 58, no. 1, pp. 140–152, 2009.
- M. L. S. Fontes, M. T. Suzuki, M. T. Cottrell, and P. C. Abreu, “Primary production in a subtropical stratified coastal lagoon-contribution of anoxygenic phototrophic bacteria,” Microbial Ecology, vol. 61, no. 1, pp. 223–237, 2011.
- H.-T. Ng, D. M. Marques, E. Jeppesen, and M. Søndergaard, “Bacterioplankton in the littoral and pelagic zones of subtropical shallow lakes,” Hidrobiologia, vol. 646, no. 1, pp. 311–326, 2010.
- A. Fonseca, E. S. Braga, and B. B. Eichler, “Distribuição espacial dos nutrientes inorgânicos dissolvidos e da biomassa fitoplanctônica no sistema pelágico da Lagoa da Conceição, Santa Catarina, Brasil (Setembro, 2000),” Atlântica, vol. 24, pp. 69–83, 2002.
- M. L. S. Fontes, R. Cavellucci, A. Laurenti, E. C. Machado, M. C. Camargo, and N. Brandini, “Detection of environmental impact on variations in dissolved nutrients and Chl-a in the Conceição Lagoon, Florianópolis, SC, Brazil,” Journal of Coastal Research, vol. 39, pp. 1407–1412, 2006.
- I. S. Maia, “Avaliação de lagoas de maturação modificadas com a presença de suportes e macrófitas da família Lemnaceae no tratamento de esgotos domésticos,” M.S. dissertation, Universidade Federal de Santa Catarina, Florianopólis, SC, Brazil, 2008.
- A. L. Fonseca, “Efeito da drenagem urbana nas características físico-químicas e biológicas da água superficial na Lagoa da Conceição (Florianópolis, SC, Brasil),” Biotemas, vol. 19, pp. 7–16, 2006.
- K. G. Porter and Y. S. Feig, “The use of DAPI for identifying and counting aquatic microflora,” Limnology and Oceanography, vol. 25, no. 5, pp. 943–948, 1980.
- R. Massana, J. M. Gasol, P. K. Bjornsen et al., “Measurement of bacterial size via image analysis of epifluorescence preparations: description of an inexpensive system and solutions to some of the most common problems,” Scientia Marina, vol. 61, no. 3, pp. 397–407, 1997.
- S. Norland, “The relationship between biomass and volume of bacteria,” in Handbook of Methods in Aquatic Microbial Ecology, P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole, Eds., Lewis Publishers, Boca Raton, FL, USA, 1993.
- C. A. Carlson and H. W. Ducklow, “Growth of bacterioplankton and consumption of dissolved organic carbon in the Sargasso Sea,” Aquatic Microbial Ecology, vol. 10, pp. 69–85, 1996.
- A. J. Underwood, Experiments in Ecology: Their Logical Design and Interpretation Using Analysis of Variance, Cambridge University Press, Cambridge, UK, 2006.
- J. H. Zar, Biostatistical Analysis, Prentice Hall, Englewood Cliffs, NJ, USA, 1996.
- J. M. Gasol, P. A. Giorgio, R. Massana, and C. M. Duarte, “Active versus inactive bacteria: size-dependence in a coastal marine plankton community,” Marine Ecology Progress Series, vol. 128, pp. 91–97, 1995.
- G. Corno and K. Jürgens, “Direct and indirect effects of protist predation on population size structure of a bacterial strain with high phenotypic plasticity,” Applied and Environmental Microbiology, vol. 72, no. 1, pp. 78–86, 2006.
- K. Jürgens and H. Gude, “The potential importance of grazing-resistant bacteria in planktonic systems,” Marine Ecology Progress Series, vol. 112, pp. 169–188, 1994.
- K. Jürgens and C. Matz, “Predation as a shaping force for the phenotypic and genotypic composition of planktonic bacteria,” Antonie van Leeuwenhoek, vol. 81, no. 1–4, pp. 413–434, 2002.
- L. Berdjeb, T. Pollet, I. Domaizon, and S. Jacquet, “Effect of grazers and viruses on bacterial community structure and production in two contrasting trophic lakes,” BMC Microbiology, vol. 11, p. 88, 2011.
- M. G. Weinbauer and F. Rassoulzadegan, “Are viruses driving microbial diversification and diversity?” Environmental Microbiology, vol. 6, no. 1, pp. 1–11, 2004.
- M. G. Weinbauer, K. Hornák, J. Jezbera, J. Nedoma, J. R. Dolan, and K. Šimek, “Synergistic and antagonistic effects of viral lysis an protistan grazing on bacterial biomass, production and diversity,” Environmental Microbiology, vol. 9, no. 3, pp. 777–788, 2007.
- D. J. Suggett, N. Stambler, O. Prásil et al., “Nitrogen and phosphorus limitation of oceanic microbial growth during spring in the Gulf of Aqaba,” Aquatic Microbial Ecology, vol. 56, pp. 227–239, 2009.
- C. J. Williams, P. J. Lavrentyev, and F. J. Jochem, “Bottom-up and top-down control of heterotrophic bacterioplankton growth in a phosphorus-depleted subtropical estuary, Florida Bay, USA,” Marine Ecology Progress Series, vol. 372, pp. 7–18, 2008.
- J. J. Cole, S. Findlay, and M. L. Pace, “Bacterial production in fresh and saltwater ecosystems: a cross-system overview,” Marine Ecology Progress Series, vol. 43, pp. 1–10, 1988.
- A. L. Cuevas, J. K. Egge, F. T. Thingstad, and B. Töpper, “Organic carbon and mineral nutrient limitation of oxygen consumption, bacterial growth and efficiency in the Norwegian Sea,” Polar Biology, vol. 34, no. 6, pp. 871–882, 2011.
- S. Drakare, P. Blomqvist, A. K. Bergstrom, and M. Jansson, “Primary production and phytoplankton composition in relation to DOC input and bacterioplankton production in humic Lake Ortrasket,” Freshwater Biology, vol. 47, no. 1, pp. 41–52, 2002.
- J. Karlsson, M. Jansson, and A. Jonsson, “Similar relationships between pelagic primary and bacterial production in clearwater and humic lakes,” Ecology, vol. 83, no. 10, pp. 2902–2910, 2002.
- D. L. Kirchman, “The uptake of inorganic nutrients by heterotrophic bacteria,” Microbial Ecology, vol. 28, no. 2, pp. 255–271, 1994.
- M. M. Mills, C. M. Moore, R. Langlois et al., “Nitrogen and phosphorus co-limitation of bacterial productivity and growth in the oligotrophic subtropical North Atlantic,” Limnology and Oceanography, vol. 53, no. 2, pp. 824–834, 2008.
- G. A. F. Flaten, T. Castberg, T. Tanaka, and F. Thingstad, “Interpretation of nutrient-enrichment bioassays by looking at sub-populations in a marine bacterial community,” Aquatic Microbial Ecology, vol. 33, pp. 11–18, 2003.
- M. L. Pace and J. J. Cole, “Comparative and experimental approaches to top-down and bottom-up regulation of bacteria,” Microbial Ecology, vol. 28, no. 2, pp. 181–193, 1994.
- R. E. Carlson, “A trophic state index for lakes,” Limnology and Oceanography, vol. 22, no. 2, pp. 361–369, 1977.
- W. K. Dodds and J. J. Cole, “Expanding the concept of trophic state in aquatic ecosystems: it’s not just the autotrophs,” Aquatic Sciences, vol. 69, no. 4, pp. 427–439, 2007.
- B. Biddanda and J. B. Cotner, “Love handles in aquatic ecosystems: the role of dissolved organic drawdown, resuspended sediments, and terrigeous inputs in the carbon balance of Lake Michigan,” Ecosystems, vol. 5, no. 5, pp. 431–445, 2002.
- B. J. Campbell, L. Yu, J. F. Heidelberg, and D. L. Kirchman, “Activity of abundant and rare bacteria in a coastal ocean,” Proceedings of the National Academy of Sciences, vol. 108, no. 31, pp. 12776–12781, 2011.
- H. P. Grossart, J. Jezbera, K. Hornak, K. M. L. Hutalle, U. Buck, and K. Simek, “Top-down and bottom-up induced shifts in bacterial abundance, production and community composition in an experimentally divided humic lake,” Environmental Microbiology, vol. 10, no. 3, pp. 635–652, 2008.
- K. Haukka, E. Kolmonen, R. Hyder et al., “Effect of nutrient loading on bacterioplankton community composition in Lake Mesocosms,” Microbial Ecology, vol. 51, no. 2, pp. 137–146, 2006.
- R. J. Newton and K. D. McMahon, “Seasonal differences in bacterial community composition following nutrient additions in a eutrophic lake,” Environmental Microbiology, vol. 13, no. 4, pp. 887–899, 2011.
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