Microencapsulation of Probiotic Strains by Lyophilization Is Efficient in Maintaining the Viability of Microorganisms and Modulation of Fecal Microbiota in Cats

Table 3

Microbial viability (log CFU/g) in feed during storage at room temperature.

Storage (day)	Treatment		SEM	value
	T1	T2		Treatment	Storage	Treatment × storage
Lactobacilli
0	6.52^a	6.05^b	0.027	<0.0001	<0.0001^1	<0.0001
7	5.99^b	5.28^c	0.027
15	5.33^c	5.26^c	0.027
30	5.13^d	4.94^e	0.027
60	4.94^e	4.97^e	0.027
90	4.44^h	4.86^f	0.027
120	4.24^i	4.76^g	0.027
Enterococci
0	6.63^a	6.28^b	0.041	<0.0001	<0.0001^2	<0.0001
7	6.32^b	5.82^d	0.041
15	5.92^cd	5.67^e	0.041
30	5.85^d	5.97^c	0.041
60	5.36^f	5.60^e	0.041
90	5.15^g	5.02^h	0.041
120	4.63^i	4.95^h	0.041
Yeasts
0	6.56^a	6.10^b	0.037	0.3030	<0.0001^3	<0.0001
7	6.17^b	5.66^d	0.037
15	5.77^c	5.43^e	0.037
30	5.15^f	5.13^f	0.037
60	5.01^g	5.01^g	0.037
90	4.69^h	5.12^f	0.037
120	4.19^i	4.94^g	0.037

Means in a column followed by different lowercase letters differ by Tukey's test (). T1: feed supplemented with a commercial probiotic product; T2: feed supplemented with freeze-dried probiotics and fructooligosaccharides; SEM: standard error of the mean. = 5.735 − 0.012x (r² = 66.99%). = 6.218 − 0.012x (r² = 86.08%). = 5.922 − 0.012x (r² = 71.17%).