International Journal of Microbiology / 2020 / Article / Fig 5

Research Article

Antibiotic Susceptibility, Clonality, and Molecular Characterization of Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii from Washington DC

Figure 5

(a) Colony PCR amplification for insertion sequence (ISAba1) gene. Upper and lower panel: Lane 1 is a control A. baumannii ATCC 19606 strain. Lanes 2-29 represent isolates A1, A2, A3, A5, A7, A8, A10, A11, A14, B1, B2, B3, B4, B8, B9, B10, B14, B16, C4, C5, C9, C11, C20, C22, C24, H4, H8, and H11, respectively. (b) PCR amplification for ISAba1 forward primer and blaOXA-51-like gene reverse primers ISAba1F/OXA-51R. Lanes 1-28 (except Lane 5) represent isolates A1, A2, A3, A5, A8, A10, A11, A14, B1, B2, B3, B4, B8, B9, B10, B14, B16, C4, C5, C9, C11, C20, C22, C24, H4, H8, and H11, respectively. The lower panel represents amplification for ISAba1 forward primer and blaOXA-23-like gene reverse primers ISAba1F/OXA-23R. Lanes 1-10 represent isolates A1, B1, B9, B14, B16, C4, C9, C24, H4, and H11, respectively.
(a)
(b)