In Vitro Antimicrobial Activity of Piper retrofractum Fruit Extracts against Microbial Pathogens Causing Infections in Human and Animals

Figure 3

Minimum inhibitory concentration (MIC) sensitivity testing was a microdilution assay using flat shape 96-well plate. This is an example of crude metabolite extract P. retrofractum Vahl against E. coli ATCC25922. The MIC was read after tetrazolium was added which has shown the lowest concentration of antimicrobial agent at which it was colorless or changed to pale pink. The wells F1 to F6 were negative control, and the wells F7 to F12 were positive control.