Research Article
Detection and Profiling of Antibiotic Resistance among Culturable Bacterial Isolates in Vended Food and Soil Samples
Table 1
List of primers for antibiotic resistance genes.
| | Primer | Sequence (5′ to 3′) | Target gene | Amplicon size (bp) | Annealing temperature (°C) | Resistance to antibiotics |
| | Flor-F | TTATCTCCCTGTCGTTCCAGCG | FloR | 450 | 57.5 | Chloramphenicol | | Flor-R | CCTATGAGCACACGGGGAGC |
| | strB-F | GGCACCCATAAGCGTACGCC | Str B | 400 | 61 | Streptomycin | | strB-R | TGCCGAGCACGGCGACTACC |
| | DfrA1-F | CGAAGAATGGAGTTATCGGG | Dfr A | 200 | 55 | Trimethoprim | | DfrA1-R | TGCTGGGGATTTCAGGAAAG |
| | TEM-F | GCGGTATCATTGCAGCACTG | BlaTEM | 1000 | 55 | Beta-lactam | | TEM-R | TGCTTAATCAGTGAGGCACC |
| | Amp-F | ATGCACACGCTGATCGGATT | Amp | 268 | 65 | Ampicillin | | Amp-R | GCGGACGCAGACTTCACTAA |
| | Tet-F | AACCGGCATTGAGAGCATCA | Tet A | 699 | 60 | Tetracycline | | Tet-R | TTGTCTCCTCTCCCTTGGCT |
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