Isolation and Identification of Some Probiotic Bacteria and Their Potential Role in Improving Immune Response and Resistance of Nile Tilapia (Oreochromis niloticus) in Comparison with a Commercial Product

Table 2

Survival, immunological parameters, and the relative level of protection of the experimental groups after one and two months of feeding experiment.

Parameter	Control		P. putida		Organic Green™
	1st month	2nd month	1st month	2nd month	1st month	2nd month
Survival %	92.55 ± 1.98^b	92.64 ± 0.10^b	98.12 ± 0.91^a	98.12 ± 0.96^a	96.23 ± 0.91^a	96.29 ± 0.96^a
Hematocrit (%)	30.6^A ± 2.09	30.2^A ± 1.81	31.0^A ± 1.89	29.6^A ± 1.62	31.0^A ± 0.98	28.8^A ± 1.03
TLC (×10^3/mL)	36.5^BC ± 1.45	40.2^A ± 0.99	40.2^A ± 0.84	37.6^AB ± 1.09	33.4^C ± 1.49	37.6^AB ± 1.09
Neutrophils	11.85^A ± 0.15	12.14^A ± 0.19	12.03^A ± 0.24	12.18^A ± 0.16	11.23^B ± 0.15	11.35^AB ± 0.14
Lymphocytes	23.4^BC ± 1.14	26.53^A ± 0.84	26.04^A ± 1.09	25.22^A ± 0.64	21.11^C ± 0.69	24.62^AB ± 0.91
Monocytes	0.90^B ± 0.12	0.92^A ± 0.13	1.41^A ± 0.16	1.12^A ± 0.14	0.98^B ± 0.17	0.95^A ± 0.14
Eosinophils	0.31^A ± 0.08	0.53^A ± 0.07	0.33^A ± 0.05	0.32^B ± 0.05	0.28^A ± 0.05	0.6^A ± 0.08
Basophils	0.04^A ± 0.04	0.83^A ± 0.06	0.11^A ± 0.06	0.12^A ± 0.06	0.12^A ± 0.06	0.09^A ± 0.06
NBT mg/ml	0.06 ± 0.01^b	0.07 ± 0.01^b	0.27 ± 0.01^a	0.25 ± 0.01^a	0.24 ± 0.00^a	0.22 ± 0.00^a
Lysozyme activity unit/ml	0.72 ± 0.05^b	1.22 ± 0.03^b	1.60 ± 0.12^a	1.84 ± 0.09^ab	1.68 ± 0.12^a	1.43 ± 0.03^b
Phagocytic index	1.30 ± 0.03^b	1.40 ± 0.01^b	1.60 ± 0.02^a	1.90 ± 0.02^a	1.67 ± 0.02^a	1.97 ± 0.03^a
Phagocytic %	22.70 ± 0.01^b	22.60 ± 0.01^b	28.90 ± 0.04^ab	30.20 ± 0.04^a	33.20 ± 0.05^a	34.20 ± 0.05^a
RLP (%)	0.0	0.0	62.5	55.5	52.5	44.4

Mean ± SE having the same letter in the same row are not significantly different at .