Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing

Figure 1

Phylogenetic tree of concatenated sequences of the 16S rRNA gene regions. The consensus sequences from the species described in Table 1 were aligned according to the variable region of 16S rRNA gene. Trees were generated using maximum likelihood, Kimura-2-parameter, gamma substitution, and bootstrap of 1000 replicates. (a) Variable regions (V1V2); (b) variable regions (V4V5); (c) variable regions (V6V7V8); (d) variable regions (V1V2 and V4V5); (e) variable regions (V1V2 and V6V7V8); (f) variable regions (V4V5 and V6V7V8); and (g) variable regions (V1V2, V4V5, and V6V7V8). The black dots indicate species that were not discriminated by specified regions to a monophyletic group.

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