Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing
Phylogenetic tree of concatenated sequences of the 16S rRNA gene regions. The consensus sequences from the species described in Table 1 were aligned according to the variable region of 16S rRNA gene. Trees were generated using maximum likelihood, Kimura-2-parameter, gamma substitution, and bootstrap of 1000 replicates. (a) Variable regions (V1V2); (b) variable regions (V4V5); (c) variable regions (V6V7V8); (d) variable regions (V1V2 and V4V5); (e) variable regions (V1V2 and V6V7V8); (f) variable regions (V4V5 and V6V7V8); and (g) variable regions (V1V2, V4V5, and V6V7V8). The black dots indicate species that were not discriminated by specified regions to a monophyletic group.