Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing

Table 2

Degenerate 16S rRNA primers for PCR assays.

16S rDNA regions	Degenerate primer sequence (5′-3′)		Tm (^oC)	Amplicon length (bases)
(V1V2)	F:	TWACACATGCAAGTCGARCG	56–59	339
	R:	CAAWATTCCCCACTGCTGCC	58–59
(V4V5)	F:	CAGCCGCGGTAATACGDAGG	60–63	380
	R:	TGCGRCCGTACTCCCCAGGC	60–63
(V6V7V8)	F:	CGA WGCAACGCGAARAACCT	60–61	362
	R:	CGAGTTGCAGACTVCAATCCG	59–62

Degenerate bases (W = A/T, R = A/G, and D = A/G/T)