Screening and Characterization of Thermostable Amylase-Producing Bacteria Isolated from Soil Samples of Afdera, Afar Region, and Molecular Detection of Amylase-Coding Gene

Table 3

Biochemical characterization of bacterial isolates.

Parameters	Bacterial isolates
Biochemical characterization	M2	M8	M13
Catalase test	+	+	+
Anaerobic growth	+	+	+
Oxidase test	+	−	+
Citrate utilization	+	−	+
Urea hydrolysis	+	+	+
Casein hydrolysis	+	+	+
Triple sugar iron test (TSI)
Carbohydrate fermentation	Only glucose fermentation	Both glucose and lactose fermentation	Only glucose fermentation
TSI (butt)	Yellow	Yellow	Yellow
TSI (slant)	Red	Yellow	Red
TSI (H2S) production	+	+	+
TSI (gas) production	+	−	+

Key: + = positive; − = negative.