Production, Optimization, and Characterization of an Acid Protease from a Filamentous Fungus by Solid-State Fermentation

Table 1

Clear zone diameter of fungal isolates on skim milk agar media.

No.	Isolates	Spore concentration/mL	CD (mm), mean ± SEM	CZD (mm), mean ± SEM	REA
1	Z1BL1	6.54 × 10^7	28.7 ± 1.5^b	21.3 ± 1.5^a	0.74
2	H1BL1	3.60 × 10^6	14.7 ± 0.8^a	9.0 ± 0.6^cd	0.61
3	Z1BL3	2.64 × 10^8	19.0 ± 1.5^a	11.7 ± 1.2^c	0,61
4	SW2W1	1.81 × 10^7	19.0 ± 2.0^a	12.3 ± 0.3^c	0.65
5	SW1BBr	8.12 × 10^7	14.3 ± 1.5^a	6.7 ± 1.5^d	0.46
6	SW1YW1^+	1.4 × 10^6	15.7 ± 1.2^a	8.3 ± 0.3^cd	0.52
7	Code 3	2.6 × 10^6	15.7 ± 0.3^a	10.0 ± 0.6^cd	0.63
8	Z1Y2	1.5 × 10^6	17.3 ± 0.8^a	7.0 ± 0.5^d	0.40
9	H1W1	1.2 × 10^6	20.3 ± 6^a	16.3 ± 1.2^b	0.80
10	SW1WY1	2.2 × 10^7	18.3 ± 2.4^a	11.7 ± 0.3^c	0.63
11	Z1BL4	4.4 × 10^8	16.7 ± 0.6^a	10.0 ± 0.6^cd	0.60
12	H1Gr1	2.6 × 10^6	15.3 ± 1.5^a	9.3 ± 0.3^cd	0.60

Mean is the average of three measurements. SEM: standard error of the mean; REA: relative enzyme activity; CD: colony diameter; CZD: clear zone hydrolysis diameter. Different letters (a, b, c, d) designate significantly different means as determined by the SPSS test .