Diclofenac May Induce PIA-Independent Biofilm Formation in Staphylococcus aureus Strains

Table 3

Degree of degradation of biofilm induced by glucose and diclofenac after treatment with sodium metaperiodate in 4 strains of S. aureus.

Strain	Control^a (OD570nm)	Glu^b		Glu + Dic^d		Dic^f
		OD570 nm	SMP^c (%)	OD570 nm	SMP^e (%)	OD570 nm	SMP^g (%)
A112	0.202	0.722	3.1	0.586	0.0	0.411	0.0
A139	0.185	0.821	0.0	0.722	0.0	0.655	0.0
A150	0.200	0.876	0.0	0.579	0.0	0.387	0.0
B103	0.192	0.642	0.0	0.602	0.0	0.625	0.0

Glu, glucose; Dic, diclofenac (50 μg/mL); SMP, sodium metaperiodate; OD, optical density. ^aStrain grown on tryptone soy broth (TSB) without supplementation; ^bstrain grown in TSB supplemented with glucose (1%); ^cdegradation by metaperiodate (%) of biofilm induced by glucose; ^dstrain grown in TSB supplemented with glucose + diclofenac; ^edegradation by metaperiodate (%) of biofilm induced by glucose + diclofenac; ^fstrain grown in TSB supplemented with diclofenac; ^gdegradation by metaperiodate (%) of biofilm induced by diclofenac.