Research Article
Epigenetic Methylation of Parathyroid CaR and VDR Promoters in Experimental Secondary Hyperparathyroidism
Table 1
PCR primers used for methylation detection by melting temperature profiling.
| | Name | Location# | 5′-3′ sequence | Size$ | Number of CpG |
| | Promoter | | | | | | CaR #1F | (−172) | AGTTTGGGAATGGTTAGAGTTTTAT | 171 bp | 12 | | CaR #1R | (−2) | ACTCCCTAAATCTCTCAAATCAAC | | CaR #2F | (−28) | AAGGTTGATTTGAGAGATTTAGG | 185 bp | 9 | | CaR #2R | (+157) | CTACTACCTCCCCrCAAACCCTCT | | VDR #1F | (−58) | TTTTTGTTTGTTAAAAGGTTGTTGT | 125 bp | 6 | | VDR #1R | (+67) | AATCTAATCACTCACCCAAAACTC | | VDR #2F | (−374) | GTTTATAGTAGATTGGGTAGAATTA | 175 bp | 12 | | VDR #2R | (−200) | CCTACCTTATAAAAAACTCTAAAATC | | VDR #3F | (−576) | GTTTTTTTGAGTTAATTTTAATTAGTGG | 169 bp | 7 | | VDR #3R | (−389) | TAATTCTACCCAATCTACTATAAAC | | VDR #4F | (−678) | AGGATTTGGGAGGTAGAGATTTTAG | 188 bp | 18 | | VDR #4R | (−510) | CCACTAATTAAAATTAACTCAAAAAAAC |
| | Control | | | | | | H19 F | (−6233) | GAGGGTAGGATATATGTATTTTTAGGTTG | 185 bp | 13 | | H19 R | (−6049) | AAAAAAATTCAATCTCAATTACAATCTATTT | | VDR DS F | (+48639) | AAGGGGTGTGAGTTTTATGTTA | 269 bp | 12 | | VDR DS R | (+48908) | AAACAATAAACATCCTATCTCCC |
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#Relative location of the 5′ nucleotide according to exon 1.
$Bp denotes DNA base pair.
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