Transcription of the Tumor Suppressor Genes p53 and RB in Lymphocytes from Patients with Chronic Kidney Disease: Evidence of Molecular Senescence?

Table 2

Expression of p53 and RB in cultured and noncultured cells.

Pairs analyzed		p53			RB
	Difference in expression	Statistical analysis; 𝑃 value/standard error range	Difference; factor	Difference in expression	Statistical analysis; 𝑃 value/standard error range	Difference; factor
A before culture—A plain culture	↓	0.000/0.013–0.541	0.131	↓	0.001/0.004–0.553	0.083
A before culture—A culture with LPS	↓	0.000/0.026–0.664	0.190	↓	0.000/0.003–0.577	0.080
A before culture—A culture with CRP	↓	0.000/0.008–0.197	0.048	↓	0.000/0.014–0.265	0.062
A before culture—A culture with LPS-LXA4	↓	0.000/0.033–0.903	0.226	↓	0.001/0.053–0.874	0.235
A before culture—B before culture	→	0.056		→	0.260
A before culture—C before culture	→	0.337		→	0.105
B before culture—B plain culture	↓	0.001/0.048–0.249	0.176	↓	0.001/0.052–0.622	0.222
B before culture—B culture with LPS	↓	0.000/0.081–0.492	0.202	↓	0.000/0.113–0.614	0.280
B before culture—B culture with CRP	↓	0.000/0.017–0.236	0.075	↓	0.000/0.030–0.408	0.097
B before culture—B culture with LPS-LXA4	↓	0.000/0.118–0.807	0.333	↓	0.002/0.157–1.267	0.460
C before culture—C plain culture	→	0.071		→	0.239
C before culture—C culture with LPS	↓	0.024/0.033–1.608	0.257	→	0.318
C before culture—C culture with CRP	↓	0.000/0.021–0.250	0.071	↓	0.000/0.060–0.515	0.180
C before culture—C culture with LPS-LXA4	↓	0.001/0.054–0.595	0.247	↓	0.050/0.188–1.753	0.500

A: healthy controls; B: CKD patients; C: Dialysis patients; these results are derived from REST software which analyzed the Ct values calculated from the real time PCR experiments.