Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β

Figure 3

Degradation of OS-9 is meprin B-specific. (a) Time-depended degradation of purified OS-9 incubated with activated purified recombinant meprin B. (b) Degradation of OS-9 was blocked by preincubation with the meprin inhibitor, actinonin. Purified OS-9 was incubated with Tris buffer only (lane 1), activated meprin B (lane 2), latent meprin B (lane 3), and activated meprin B preincubated with the meprin inhibitor actinonin (lane 4). Degradation of OS-9 was not observed with the latent form of meprin B. Degradation of OS-9 was also blocked by preincubation of meprin B with the meprin inhibitor, actinonin.

(a)

(b)