Proteasome Activators, PA28α and PA28β, Govern Development of Microvascular Injury in Diabetic Nephropathy and Retinopathy
PA28α and PA28β regulate the expression of OPN in cultured mesangial cells under high glucose concentrations. (a) Western blot analysis of the conditioned media of cultured wild type and Pa28αβDKO mesangial cells under different glucose conditions and osmolarity treatment. OPN: Osteopontin and FN: Fibronectin. (b) qPCR analysis of OPN and (c) MCP-1 mRNA in wild type and Pa28αβDKO mesangial cells under different glucose concentrations. (d) Western blot analysis for OPN in the conditioned media and PA28α, PA28β, and β-actin in the cell lysate following lentiviral reexpression of PA28α and PA28α/PA28β in Pa28αβDKO mesangial cells. Results presented as mean ± SEM. .
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