Research Article

Bayesian Estimation of the Axial Position in Astigmatism-Based Three-Dimensional Particle Tracking

Figure 1

Variation in the lateral bead profile with axial position. (a) Five images of the same 57 nm beam taken at different axial positions demonstrate the astigmatism. (b) The - and -widths ( : red +, : blue ) for each height calculated by fitting the bead image to (1). Solid lines are a fit of the width data to the defocusing curve described in (5). (c) Two-dimensional width plot of the same data plotted in (b). Color scale represents the -position. Letters denote the location of points in the ( ) plane analyzed and tabulated in Table 1. The solid line is data from the same fit as for (b).
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(a)
896208.fig.001b
(b)
896208.fig.001c
(c)