Bayesian Estimation of the Axial Position in Astigmatism-Based Three-Dimensional Particle Tracking
Figure 1
Variation in the lateral bead profile with axial
position. (a) Five images of the same 57 nm beam taken at different axial
positions demonstrate the astigmatism. (b) The - and -widths (: red +, : blue ) for each
height calculated by fitting the bead image to (1). Solid lines are a fit of
the width data to the defocusing curve described in (5). (c) Two-dimensional
width plot of the same data plotted in (b). Color scale represents the -position.
Letters denote the location of points in the () plane analyzed and tabulated in Table 1. The solid
line is data from the same fit as for (b).