Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber

Figure 2

(a) Arrows point to the position of the set screws that need to be adjusted to keep the chuck aligned along the vertical axis (Left). Undesired trajectory of optical fiber resulting from improper set screw adjustment (Right). (b) Fluctuations caused by the rotary joint (left) introduce some nonuniformity in fluorescence intensity recording which in this case shows a spike at 150 degrees. To eliminate the effect, this nonuniformity was measured in a uniform fluorescence solution. Next, the complement of this data was created as a distortion compensation matrix (Right) and this matrix was used to cancel the effect.