Math1 transfection increases the mucous cells in the middle ear mucosa of mice. Full-length Math1 cDNA was cloned into a protein-expressing vector (C2, pEGFP, Clontech). To study the role of Math1 in the middle ear mucosa, bilateral bullae of 5 mice were transfected with 10 μL of Math1 and empty vectors, respectively, at 1.4 μg/mL in Opti-MEM containing Lipofectin at 6 μg/mL via the tympanic membrane approach. Transfected animals were sacrified 7 days after Math1 transfection for harvest of the bullae. Math1 transfection had more AB-PAS positive cells in the mouse middle ear mucosa (upper) than empty vector (C2) transfection (lower). Bar = 10 μm.
