More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis

Figure 1

Chromatographic profile of the purified fractionated sinus mucin sample (S1). Sepharose CL-2B gel column (125 × 2.5 cm) was eluted by upward flow with 0.2 M sodium chloride containing 0.02% (w/v) sodium azide and flow rate 18 mL/h. Calibration was firstly performed using 1% (w/v) dextran blue solution containing 0.05% (w/v) methyl orange. The glycoprotein content was estimated by the PAS solution assay. and are the void and total volumes, respectively.