More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis
Table 1
Amino acid composition of native/polymeric chronic sinusitis mucins.
Amino acid
S1
S2
S3
S4
S5
S6
S7
S8
MUC5AC rich samples
MUC2 rich samples
% content of amino acids
% content of amino acids
His
5.7 ± 0.2
9.9 ± 0.1
6.3 ± 0.7
8.5 ± 0.4
11.0 ± 1.8
1.8 ± 0.4
3.1 ± 0.1
4.1 ± 0.1
Arg
14.9 ± 1.9
16.8 ± 1.8
11.5 ± 1.4
14.6 ± 1.0
6.6 ± 0.6
13.7 ± 1.6
11.2 ± 1.7
24.5 ± 0.5
Ser
14.7 ± 0.2
18.5 ± 1.3
21.0 ± 2.1
12.2 ± 1.3
16.7 ± 2.3
35.7 ± 2.7
27.4 ± 3.5
23.7 ± 0.5
Thr
25.2 ± 0.7
33.2 ± 3.2
35.8 ± 0.8
24.6 ± 0.5
12.1 ± 0.5
25.2 ± 1.1
17.4 ± 1.8
20.1 ± 1.1
Asp
32.3 ± 0.3
19.5 ± 0.3
22.8 ± 0.2
21.3 ± 1.2
12.5 ± 1.5
11.4 ± 0.4
7.3 ± 1.0
6.2 ± 0.2
Glu
23.9 ± 0.4
19.5 ± 1.3
23.5 ± 0.4
12.8 ± 0.9
20.0 ± 1.2
15.6 ± 1.4
7.8 ± 1.2
10.3 ± 0.8
Gly
12.4 ± 0.1
7.8 ± 0.1
13.3 ± 1.2
7.2 ± 0.2
10.3 ± 0.4
3.9 ± 0.9
6.8 ± 0.4
5.3 ± 0.7
Ala
18.1 ± 0.8
23.6 ± 0.6
25.0 ± 0.9
31.0 ± 3.4
12.2 ± 1.4
22.9 ± 2.7
18.0 ± 1.2
19.1 ± 0.1
Pro
21.2. ± 2.8
26.9 ± 1.9
32.3 ± 3.1
27.9 ± 0.1
17.7 ± 0.3
23.1 ± 1.6
15.4 ± 2.3
12.8 ± 0.8
Val
9.5 ± 0.5
12.3 ± 0.3
16.5 ± 0.4
9.7 ± 0.7
7.2 ± 0.7
7.0 ± 0.8
6.6 ± 0.4
3.0 ± 1.1
Ile/Leu/Phe
16.4 ± 0.2
16.6 ± 0.7
23.8 ± 0.1
18.2 ± 0.8
15.0 ± 1.8
11.2 ± 1.3
15.6 ± 1.7
9.7 ± 2.3
Lys
13.0 ± 1.3
11.9 ± 1.8
16.5 ± 1.4
5.0 ± 1.5
11.6 ± 1.5
3.9 ± 0.2
5.8 ± 1.2
4.1 ± 0.9
Calc. ptn
207.3 ± 1.1
216.5 ± 4.5
248.3 ± 5.6
193.0 ± 3.6
152.9 ± 8.3
175.4 ± 14.3
142.2 ± 8.2
142.9 ± 4.0
The values quoted are of the % amino acid contents in polymeric mucins. The samples were arranged according to the proportions of the relevant predominant mucin. In the first 4 samples MUC5AC (S1–S4) was predominant and MUC2 was predominant in the other 4 samples (S5–S8). Amino acid content was calculated in quadruplicate samples and the mean content of amino acid in each sample was calculated. Calc. ptn: calculated total protein in ug/mg of freeze-dried mucin.
