More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis
Table 2
Amino acid composition of reduced chronic sinusitis mucins.
Amino acid
S1
S2
S3
S4
S5
S6
S7
S8
MUC5AC rich samples
MUC2 rich samples
% content of amino acids
% content of amino acids
His
3.8 ± .0.7
1.6 ± 0.4
2.1 ± 0.1
5.5 ± 0.4
7.8 ± 0.7
2.3 ± 0.3
3.5 ± 0.1
1.5 ± 0.3
Arg
20.4 ± 1.4
13.2 ± 1.7
7.2 ± 0.5
9.7 ± 0.7
4.2 ± 0.2
9.9 ± 1.0
5.9 ± 0.4
7.3 ± 0.7
Ser
27.9 ± 0.1
12.4 ± 1.3
22.5 ± 1.5
14.3 ± 0.7
11.1 ± 1.1
27.8 ± 0.8
25.5 ± 1.6
18.6 ± 1.6
Thr
30.6 ± 0.5
25.4 ± 2.4
32.9 ± 1.2
25.8 ± 2.1
21.2 ± 0.2
19.4 ± 1.2
17.8 ± 0.8
9.4 ± 0.4
Asp
32.4 ± 0.4
19.7 ± 1.6
30.5 ± 2.2
20.6 ± 0.6
11.2 ± 0.3
7.2 ± 0.2
4.9 ± 0.4
20.2 ± 1.6
Glu
9.6 ± 1.4
9.8 ± 0.2
21.2 ± 1.2
11.1 ± 1.0
17.2 ± 0.2
8.2 ± 1.3
8.3 ± 0.3
4.5 ± 0.6
Gly
5.7 ± 1.2
6.8 ± 0.4
17.0 ± 1.3
14.5 ± 0.8
3.4 ± 0.2
6.6 ± 0.6
7.0 ± 0.2
1.0 ± 0.1
Ala
25.2 ± 1.0
22.0 ± 2.2
24.2 ± 1.1
24.2 ± 1.9
5.8 ± 0.8
14.7 ± 1.2
15.4 ± 1.5
10.7 ± 0.1
Pro
26.5 ± 2.7
27.9 ± 0.9
24.8 ± 2.2
16.1 ± 1.7
9.5 ± .0.5
24.2 ± 1.2
11.3 ± 0.2
11.4 ± 0.3
Val
4.6 ± 0.6
3.4 ± 0.6
9.1 ± 0.1
3.7 ± 0.2
2.9 ± 0.3
5.3 ± 0.3
4.6 ± 0.8
1.6 ± 0.4
Ile/Leu/Phe
14.6 ± 1.8
0.9 ± 1.5
11.0 ± 1.1
13.0 ± 1.2
6.9 ± 0.3
15.1 ± 1.3
20.8 ± 0.8
13.7 ± 1.5
Lys
5.4 ± 1.0
5.5 ± 0.4
7.6 ± 1.1
2.6 ± 0.2
8.8 ± 0.2
2.9 ± 0.5
3.6 ± 0.7
00 ± 00
Calc. ptn
206.7 ± 10.2
156.7 ± 9.9
210.0 ± 13.4
161.1 ± 3.8
110.0 ± 2.2
143.6 ± 3.7
128.0 ± 3.4
99.7 ± 7.6
The values quoted are of the % amino acid contents in reduced sinus mucins. The samples were arranged according to the proportions of the relevant predominant mucin. In the first 4 samples MUC5AC (S1–S4) was predominant and MUC2 was predominant in the other 4 samples (S5–S8). Amino acid content was calculated in quadruplicate samples and the mean content of amino acid in each sample was calculated. Calc. ptn: calculated total protein in ug/mg of freeze-dried mucin.
