Low-Dose UVA Radiation-Induced Adaptive Response in Cultured Human Dermal Fibroblasts
Figure 2
Morphological changes in cultured fibroblasts recorded by light microscopy. (a) SIG, normal fibroblasts showed active cell proliferation. (b) LDG1, single irradiation of 7.2 J/cm2 UVA caused no significant effect on cell morphology and cell proliferation. (c) LDG5, repetitive irradiation of 7.2 J/cm2 UVA at a total dose of 115.2 J/cm2 caused large cell volume, increased intracellular particles, and inhibited cell proliferation. (d) HDG, 12 h after 86.4 J/cm2 UVA irradiation: massive apoptotic death was observed. (e) ARG1, 3 h after single low-dose UVA preirradiation: atomic and death of fibroblasts caused by sequential 86.4 J/cm2 UVA irradiation was reduced. (f) ARG5, 3 h after repetitive irradiation of 7.2 J/cm2 UVA at a cumulative dosage of 115.2 J/cm2, death of fibroblasts caused by subsequent 86.4 J/cm2 UVA irradiation was reduced significantly.
