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International Journal of Photoenergy
Volume 2012, Article ID 615259, 8 pages
Research Article

Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma

1Departamento de Cirurgia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, SP, Brazil
2Instituto de Pesquisa e Desenvolvimento—IP&D, Universidade do Vale do Paraíba (UNIVAP), Avenida Shishima Hifumi 2911, 12244-000 São José dos Campos, SP, Brazil
3Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São Carlense 400, 13566-590 São Carlos, SP, Brazil

Received 13 October 2011; Revised 7 December 2011; Accepted 9 December 2011

Academic Editor: Xie Quan

Copyright © 2012 Raduan Hage et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study aimed to investigate the pharmacokinetics of a hematoporphyrin derivative in colonic tumors induced by dimethylhydrazine and adjacent normal colon in Wistar rats using an in vivo fluorescence spectroscopy technique. In conventional clinical application of photodynamic therapy, the interval between photosensitizer (PS) administration and lesion illumination is often standardized without taking into account variations due to the type or localization of the tumor and intrinsic differences in the microcirculation and vascular permeability of each target organ. The analysis of the fluorescence spectra was based on the intensity of porphyrin emission band centered at around 620 nm in normal colon and colon tumors. The photosensitizer fluorescence intensity rapidly grew for carcinoma and normal colon, reaching the maximum values 1 and 3 hours after PS injection, respectively. Data presented here allow us to verify that the best compromise between selectivity and drug concentration for colon carcinoma in rats took place in the interval between 1 to 4 h after PS injection.