Autoregulatory Feedback Mechanism of P38MAPK/Caspase-8 in Photodynamic Therapy-Hydrophilic/Lipophilic Tetra--(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells
Figure 4
Effect of TαPcZn-PDT on Caspase-3, Bcl-2, and Bid, respectively, without or with SB203580 or Ac-IEFD-CHO in TαPcZn-PDT-induced apoptosis of Bel-7402 cells analyzed by immunoblot assay. Bel-7402 cells were pretreated with TαPcZn (54 μM) in the absence or presence of SB203580 (10 μM) or Ac-IEFD-CHO (10 μM) for 2.5 h, exposed to red-light irradiation (53.7 J/cm2), and then incubated for 3 h. Expression of cleaved Caspase-3, Bcl-2, and Bid was analyzed by immunoblot assay.
