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International Journal of Photoenergy
Volume 2014 (2014), Article ID 205839, 8 pages
http://dx.doi.org/10.1155/2014/205839
Research Article

Stimulative Effects of Low Intensity He-Ne Laser Irradiation on the Proliferative Potential and Cell-Cycle Progression of Myoblasts in Culture

1Department of Wound Healing and Cell Biology, Burns Institute, The First Affiliated Hospital, General Hospital of Chinese People’s Liberation Army, Beijing 100048, China
2Institute of Traditional Chinese Medicine, General Hospital of Chinese People’s Liberation Army, Beijing 100853, China
3Department of Pharmacy, General Hospital of Beijing Military Region, Beijing 100700, China
4Department of Ophthalmology, Second Artillery Gerenal Hospital of Chinese People’s Liberation Army, Beijing 100088, China
5Department of Electromagnetic and Laser Biology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China

Received 17 April 2014; Accepted 10 June 2014; Published 21 July 2014

Academic Editor: Timon Cheng-Yi Liu

Copyright © 2014 Cui-Ping Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Low intensity laser irradiation (LILI) was found to promote the regeneration of skeletal muscle in vivo but the cellular mechanisms are not fully understood. Myoblasts, normally quiescent and inactivated in adult skeletal muscle, are a type of myogenic progenitor cells and considered as the major candidates responsible for muscle regeneration. The aim of the present study was to study the effect of LILI on the growth potential and cell-cycle progression of the cultured myoblasts. Primary myoblasts isolated from rat hind legs were cultured in nutrient-deficient medium for 36 hours and then irradiated by helium-neon laser at a certain energy density. Immunohistochemical and flow cytometric analysis revealed that laser irradiation could increase the expression of cellular proliferation marker and the amount of cell subpopulations in the proliferative phase as compared with the nonirradiated control group. Meanwhile, the expressions of cell-cycle regulatory proteins in the laser-treated myoblasts were markedly upregulated as compared to the unirradiated cells, indicating that LILI could promote the reentry of quiescent myoblasts into the cell division cycle. These results suggest that LILI at certain fluences could promote their proliferation, thus contributing to the skeletal muscle regeneration following trauma and myopathic diseases.