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International Journal of Photoenergy
Volume 2014 (2014), Article ID 384816, 8 pages
http://dx.doi.org/10.1155/2014/384816
Research Article

Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle

1Laboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou 510006, China
2School of Physical Education and Health, Zhaoqing University, Zhaoqing 526061, China
3Teaching Department of Ideological and Political Theory, Zhaoqing University, Zhaoqing 526061, China
4Institute of Molecular Medicine and Genetics, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA

Received 27 February 2014; Revised 23 May 2014; Accepted 24 May 2014; Published 19 June 2014

Academic Editor: Hongtao Yu

Copyright © 2014 Fang-Hui Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups ( per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly ( ) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group ( ). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.