Review Article

Neuronal Differentiation of Adipose Derived Stem Cells: Progress So Far

Table 2

Laser parameters used in stem cell studies.

StudyLaser parametersResultsReferences

Human dental pulp stem cell (hDPSC) and LILI20 mW/6 s, 40 mW/3 s, 660 nmIncreased viability and proliferation[55]
ADSCS and LILI0.153 W/cm2 for 30, 45, 60, 180, and 300 s
532 nm
Increased proliferation at 30–60 s,
longer exposure decreased proliferation, and autofluorescence
[56]
Neuronal embryonic rat brain cells and LILI10, 30, 50, 110, 160, 200, and 250 mW for 1, 4, or 7 min, 780 nmIncreased migration and fiber sprouting, large size neurons with dense branched interconnected network of neuronal fibers[57]
Primary neuronal culture and LILI50 mW, 0.2, 4, 10, and 30 J/cm2, 808 nmInduced neuronal growth and neurite extension alter microglial phenotype[58]
Human neural progenitor (NHNP) and LILI50 mW/cm2, 0.05 J/cm2
808 nm
Increased ATP production[59]
ADSCs and LILI5 J/cm2, 635 nmIncreased viability and proliferation[60]
ADSCs and LILI5 J/cm2, 636 nmIncreased viability and proliferation[61]
ADSCs and LILI10 and 15 J/cm2
830 nm
Decreased viability and proliferation[62]
ADSCsNo LILIIncreased viability, proliferation, and differentiation[63]
Human fibroblasts and primary rat cortical neurons and LILI1 W, 10 mW/cm2, 0.01, 0.1, 0.5, 2, 10, 50, 200, 1,000, 5,000 mJ/cm2 810 and 980 nmImproved neurite elongation, accelerated nerve regeneration, and improved functional recovery[64]
ADSCs and LILI5 J/cm2, 636 nmADSC differentiation into smooth muscle cells[63]
ADSCsNo LILIADSC differentiation into smooth muscle cells[65]
ADSCs transplanted in ischemic mouse limbsNo LILIEnhancement of angiogenesis and osteogenesis[66, 67]
ADSCsNoDifferentiation into smooth muscle cells[68]