Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells

Figure 4

Opening of KATP channels by photobiomodulation in a Kir6.2-transfected HEK-293 cell. The current was initially recorded in the cell-attached configuration. The patch membrane was excised at the time marked Inside out. The cell was bathed in extracellular solution, and the patch membrane was held at 0 mV throughout the recording period. ATP was applied at the concentrations indicated by the bars above the current trace. Irradiation indicates the period during which the patched cell was exposed to photobiomodulation (see text for details of photobiomodulation treatment). In this and subsequent figures, the dotted line through the current recording indicates the closed channel level.