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International Journal of Photoenergy
Volume 2015 (2015), Article ID 916838, 9 pages
Research Article

Effect of 660 nm Light-Emitting Diode on the Wound Healing in Fibroblast-Like Cell Lines

1Department of Orthopedic Surgery, Chonnam National University, College of Medicine, Gwangju 501-757, Republic of Korea
2LED Test & Measurement Technology Center, KOPTI, Gwangju 500-779, Republic of Korea
3Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University, Gwangju 501-757, Republic of Korea
4Department of Environmental Engineering, Sunchon National University, Suncheon, Jeonnam 540-742, Republic of Korea
5Department of Dental Materials, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea

Received 7 July 2015; Accepted 14 September 2015

Academic Editor: Nai Ki Mak

Copyright © 2015 Myung-Sun Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Light in the red to near-infrared (NIR) range (630–1000 nm), which is generated using low energy laser or light-emitting diode (LED) arrays, was reported to have a range of beneficial biological effects in many injury models. NIR via a LED is a well-accepted therapeutic tool for the treatment of infected, ischemic, and hypoxic wounds as well as other soft tissue injuries in humans and animals. This study examined the effects of exposure to 660 nm red LED light at intensities of 2.5, 5.5, and 8.5 mW/cm2 for 5, 10, and 20 min on wound healing and proliferation in fibroblast-like cells, such as L929 mouse fibroblasts and human gingival fibroblasts (HGF-1). A photo illumination-cell culture system was designed to evaluate the cell proliferation and wound healing of fibroblast-like cells exposed to 600 nm LED light. The cell proliferation was evaluated by MTT assay, and a scratched wound assay was performed to assess the rate of migrating cells and the healing effect. Exposure to the 660 nm red LED resulted in an increase in cell proliferation and migration compared to the control, indicating its potential use as a phototherapeutic agent.