Efficacy of 670 nm Light Therapy to Protect against Photoreceptor Cell Death Is Dependent on the Severity of Damage

Figure 5

Effect of 9 J/cm² of 670 nm light on the mitochondrial respiration of 661 W photoreceptor cells. (a) Under light damage conditions, the cells exhibited an increase in both spare respiratory capacity (SRC) and nonmitochondrial respiration (NMR) when treated with 670 nm light. ATP production (ATP), proton leak (PL), and basal respiration (BR) did not change between groups ( for both groups; “” denotes significance, ; error bars represent SEM). (b) 670 nm light did not produce a difference in relative basal ECAR measurements in both normal and light damaged conditions. Light damage (LD) significantly decreased the ECAR measurements in cells, both treated and nontreated, when compared to nonlight damaged cells ( for nonlight damaged cells; for light damaged cells; “” denotes significance using one-way ANOVA with Tukey’s post hoc test, ; error bars represent SEM).

(a) Mitochondrial respiration

(b) Relative glycolytic rate