Functional Nanohybrid Materials from Photosynthetic Reaction Center Proteins

Figure 6

Representative examples of transient absorption and grating signal changes on a suspension of quinone-depleted (NoQ) RC and of a quinone-reconstituted RC; the RCs were purified from carotenoidless purple bacterium Rb. sphaeroides R-26. For comparison, the TA signal of quinone-depleted carotenoid-containing Rb. sphaeroides 2.4.1. RC is also plotted. TA was measured at two wavelengths (771 and 860 nm) as indicated and described in [43, 72]. TG was measured as described in [20]. Reaction schemes for the primary charge separation PQ → P⁺(Q_AQ_B)⁻, interquinone electron transfer P⁺(Q_A⁻Q_B ⇆ Q_AQ⁻), and the charge recombination P⁺BPheo⁻ → PBPheo and P⁺(Q_AQ_B)⁻ → PQ_AQ_B are also indicated. TA and TG signals are normalized to the maximum values.