Predominant Dissemination of PVL-Negative CC89 MRSA with SCCmec Type II in Children with Impetigo in Japan

Table 1

List of primers.

Gene	Primer name	Size of product (bp)	Primer sequence (5′→3′)	Reference
(1) Detection of virulence gene				[26]
lukS-PV-lukF-PV	luk-PV-1		ATCATTAGGTAAAATGTCTGGACATGATCCA
	luk-PV-2	433	GCATCAASTGTATTGGATAGCAAAAGC
tst	TST-1		TTCACTATTTGTAAAAGTGTCAGACCCACT
	TST-2	180	TACTAATGAATTTTTTTATCGTAAGCCCTT
eta	mpETA-1		ACTGTAGGAGCTAGTGCATTTGT
	mpETA-3	190	TGGATACTTTTGTCTATCTTTTTCATCAAC
etb	mpETB-1		CAGATAAAGAGCTTTATACACACATTAC
	mpETB-2	621	AGTGAACTTATCTTTCTATTGAAAAACACTC
(2) SCCmec typing				[28]
(i) ccr gene complex type with mecA
mecA	mA1		TGCTATCCACCCTCAAACAGG
	mA2	286	AACGTTGTAACCACCCCAAGA
Type 1	𝛼1		AACCTATATCATCAATCAGTACGT
	𝛽c	695	ATTGCCTTGATAATAGCCITCT
Type 2	𝛼2		TAAAGGCATCAATGCACAAACACT
	𝛽c	937	ATTGCCTTGATAATAGCCITCT
Type 3	𝛼3		AGCTCAAAAGCAAGCAATAGAAT
	𝛽c	1,791	ATTGCCTTGATAATAGCCITCT
Type 4	𝛼4.2		GTATCAATGCACCAGAACTT
	𝛽4.2	1,287	TTGCGACTCTCTTGGCGTTT
type 5	γF		CGTCTATTACAAGATGTTAAGGATAAT
	γR	518	CCTTTATAGACTGGATTATTCAAAATAT
(ii) mec gene complex class
mecA	ml6		CATAACTTCCCATTCTGCAGATG
	mA7	1,963	ATATACCAAACCCGACAACTACA
mecB	IS7		ATGCTTAATGATAGCATCCGAATG
	mA7	2,827	ATATACCAAACCCGACAACTACA
mecC (C2)	mA7		ATATACCAAACCCGACAACTACA
	IS2(iS-2)	804	TGAGGTTATTCAGATATTTCGATGT
(iii) SCCmec type II subtyping
IIa (II.1)	kdpB2		TAAACTGTGTCACACGATCCAT
	kdpB1	287	GATTACTTCAGAACCAGGTCAT
IIb (II.2)	2b3		GCTCTAAAAGTTGGATATGCG
	2b4	1,518	TGGATTGAATCGACTAGAATCG
IIE (II.3)	4b3		AACCAACAGTGGTTACAGCTT
	4b4	726	CGGATTTTAGACTCATCACCAT
II.4	II4-3		GTACCGCTGAATATTGATAGTGAT
	II4-1	2,003	ACTCTAATCCTAATCACCGAAC
(iv) SCCmec type IV subtyping
IVa (IV.1)	4al		TTTGAATGCCCTCCATGAATAAAAT
	4a3	458	AGAAAAGATAGAAGTTCGAAAGA
IVb (IV.2)	4b3		AACCAACAGTGGTTACAGCTT
	4b4	726	CGGATTTTAGACTCATCACCAT
IVc (IV.3)	4c5		ATCCATTTCTCAGGAGTTAG
	4c4	259	AGGAAATCGATGTCATTATAA
IVd (IV.4)	4d3		AATTCACCCGTACCTGAGAA
	4d4	1,242	AGAATGTGGTTATAAGATAGCTA
(3) Multilocus sequence typing (MLST)				[27]
carbamate kinase (arcC)	arcC-Up		TTGATTCACCAGCGCGTATTGTC
	arcC-Dn	456	AGGTATCTGCTTCAATCAGCG
Shikimate dehydrogenase (aroE)	aroE-Up		ATCGGAAATCCTATTTCACATTC
	aroE-Dn	456	GGTGTTGTATTAATAACGATATC
Glycerol kinase (glpF)	glpF-Up		CTAGGAACTGCAATCTTAATCC
	glpF-Dn	465	TGGTAAAATCGCATGTCCAATTC
Guanylate kinase (gmk)	gmk-Up		ATCGTTTTATCGGGACCATC
	gmk-Dn	429	TCATTAACTACAACGTAATCGTA
Phosphate acetyltransferase (pta)	pta-Up		GTTAAAATCGTATTACCTGAAGG
	pta-Dn	474	GACCCTTTTGTTGAAAAGCTTAA
Triosephosphate isomerase (tpi)	tpi-Up		TCGTTCATTCTGAACGTCGTGAA
	tpi-Dn	402	TTTGCACCTTCTAACAATTGTAC
Acetyl coenzyme A acetyltransferase (yqiL)	yqiL-Up		CAGCATACAGGACACCTATTGGC
	yqiL-Dn	516	CGTTGAGGAATCGATACTGGAAC