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International Journal of Pediatrics
Volume 2014, Article ID 314316, 4 pages
Research Article

Genotyping of Methicillin Resistant Staphylococcus aureus Strains Isolated from Hospitalized Children

1Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives “LR99ES27”, Faculté de Pharmacie de Monastir, Avenue Avicenne, 5000 Monastir, Tunisia
2Laboratoire de Microbiologie, CHU Fattouma Bourguiba, 5000 Monastir, Tunisia

Received 20 July 2014; Accepted 30 September 2014; Published 22 October 2014

Academic Editor: Francesco Porta

Copyright © 2014 Mouna Ben Nejma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Community associated methicillin resistant Staphylococcus aureus (CA-MRSA) is an emerging pathogen increasingly reported to cause skin and soft tissue infections for children. The emergence of highly virulencet CA-MRSA strains in the immunodeficiency of young children seemed to be the basic explanation of the increased incidence of CA-MRSA infections among this population. The subjects of this study were 8 patients hospitalized in the Pediatric Department at the University Hospital of Monastir. The patients were young children (aged from 12 days to 18 months) who were suffering from MRSA skin infections; two of them had the infections within 72 h of their admission. The isolates were classified as community isolates as they all carried the staphylococcal cassette chromosome mec (SCCmec) IV and pvl genes. Epidemiological techniques, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), were applied to investigate CA-MRSA strains. Analysis of molecular data revealed that MRSA strains were related according to PFGE patterns and they belonged to a single clone ST80. Antimicrobial susceptibility tests showed that all strains were resistant to kanamycin and 2 strains were resistant to erythromycin.