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International Journal of Pediatrics
Volume 2014, Article ID 636238, 8 pages
http://dx.doi.org/10.1155/2014/636238
Research Article

Interplay of T Helper 17 Cells with CD4+CD25high FOXP3+ Tregs in Regulation of Allergic Asthma in Pediatric Patients

1Advanced Pediatric Centre, Post Graduate Institute of Medical Education and Research, Sector 12, Chandigarh 160012, India
2Department of Natural Science, West Bengal University of Technology, Kolkata 700064, India
3Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India

Received 3 March 2014; Revised 6 May 2014; Accepted 11 May 2014; Published 4 June 2014

Academic Editor: Emmanuel Katsanis

Copyright © 2014 Amit Agarwal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. There is evidence that Tregs are important to prevent allergic diseases like asthma but limited literature exists on role of cells in allergic diseases. Methods. Fifty children with asthma and respiratory allergy (study group) and twenty healthy children (control group) were recruited in this study. Total IgE levels and pulmonary function tests were assessed. The expression of Tregs and cytokines was determined by flow cytometry. Results. The average level of total IgE in study group (316.8 ± 189.8 IU/mL) was significantly higher than controls (50 ± 17.5 IU/mL, ). The frequency of cells and culture supernatant level of IL-17 in study group (12.09 ± 8.67 pg/mL) was significantly higher than control group (2.01 ± 1.27 pg/mL, ). Alternatively, the frequency of FOXP3 level was significantly lower in study group [(49.00 ± 13.47)%] than in control group [(95.91 ± 2.63)%] and CD4+CD25+FOXP3+ to CD4+CD25+ ratio was also significantly decreased in study group [(6.33 ± 2.18)%] compared to control group [(38.61 ± 11.04)%]. The total serum IgE level is negatively correlated with FOXP3 level ( , ). The FOXP3 expression is negatively correlated with the IL-17 levels ( , ) and IL-4 levels ( , ). Conclusions. Imbalance in /Tregs, elevated IL-17, and IL-4 response and downregulation of FOXP3 were associated with allergic asthma.