Easy and Rapid Purification of Highly Active Nisin
Figure 1
Purification of nisin via cation exchange chromatography. The elution profiles of the purification of commercial nisin (a) and nisin produced by L. lactis NZ9700 (b) are shown. In both cases, nisin is eluted from the column using a five-step gradient with 200 mM (Step I), 400 mM (Step II), 600 mM (Step III), 800 mM (Step IV) and 1 M NaCl (Step V). The different elution steps and corresponding NaCl concentrations are indicated by the dashed line and the right -axis, respectively. Protein was detected by measuring the absorbance at 215 nm.
