Easy and Rapid Purification of Highly Active Nisin

Table 1

𝐼𝐶₅₀ values of each nisin containing fraction eluted from the cIEX column. Values are combined data from at least three independent nisin purifications and subsequent inhibition experiments.

	Purchased nisin		NZ9700 secreted nisin
Elution step	IC50 (nM)	Yield (mg)	IC50 (nM)	Yield (mg)
Step I (200 mM)	35.1±0.1	4.2	n.i.	0.06
Step II (400 mM)	2.6±0.1	15.1	11.2 ± 0.3	5.90
Step III (600 mM)	6.9±0.2	3.8	n.i.	3.29
Step IV (800 mM)	27.0±0.2	1.1	n.i.	0.14
Step V (1 M)	n.i.	0.8	n.i.	0.45

n.i = no inhibition observed under the experimental setup.