Research Article
Ghrelin is an Osteoblast Mitogen and Increases Osteoclastic Bone Resorption In Vitro
Table 1
Ghrelin receptor expression in bone.
| This study | Method | Result |
| Primary tissue | | | Human osteoblasts | Quantitative PCR | 3/10 positive | Human bone marrow | | 4/16 positive | Mouse bone marrow | | Negative | Rat osteoblastic cells | | Negative | Mouse hypothalamus | | Positive | Cell lines | | | Mouse osteoblastic MC3T3-E1 | | Negative | Mouse macrophage RAW264.7 | | Negative |
| Kim et al. [23] | | |
| Primary Tissue | | | Rat osteoblastic cells | Western blot | Positive | Mouse osteoblastic cells | Rat brain | Cell lines | | | Mouse osteoblastic MC3T3-E1 | | Positive | Mouse preadipocytic 3T3-L1 | RT-PCR, Western blot | Rat ROS17/2.8 osteogenic sarcoma | Rat UMR-106 osteogenic sarcoma | |
| Maccarinelli et al. [24] | | |
| Primary Tissue | | | Rat osteoblastic cells | RT-PCR, Western blot | Positive |
| Ueberberg et al. [25] | | |
| Primary Tissue | | | Human bone marrow | Quantitative PCR, IHC | Positive |
| Delhanty et al. [27] | | |
| Primary Tissue | | | Human bone marrow | Quantitative PCR | Positive |
| Fukushima et al. [28] | | |
| Primary Tissue | | | Rat osteoblastic cells | RT-PCR, IHC | Positive | Rat femur | IHC | Positive | Cell lines | | | Rat UMR-106 osteogenic sarcoma | RT-PCR | Positive |
| Guan et al. [35] | | |
| Primary Tissue | | | Rat bone marrow | RPA | Negative |
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RT-PCR: reverse transcription PCR. IHC: immunohistochemistry. RPA, ribonuclease protection assay.
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