Research Article
Leptin and Fasting Regulate Rat Gastric Glucose-Regulated Protein 58
Table 2
Cutoff score value for protein: 71 (score is −10Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant ()). The software used for peak-picking was 4000 Series Explorer (TM) RAC Software, version 3.5.3 (Applied Biosystems/MDS SCIEX, Concord, Ontario, Canada). Parameters and thresholds used for peak-picking: (a) intensity or S/N threshold: S/N = 10, local noise window width (m/z) = 250, min peak width at full width half max (bins) = 2.9; (b) means of calibrating each spectrum: internal calibration with peptides from trypsin autolysis (M + H+ = 842.509, M + H+ = 2211.104); (c) resolution: 12000 for the mass 842.51 and 18000 for the mass 2211. Search parameters: (a) MASCOT (Matrix Science, London, UK) software, VERSION 2.0; (b) enzyme specificity: trypsin; (c) missed cleavages permitted: 1; (d) fixed modification (s): carbamidomethyl (C); (e) variable modifications: oxidation (M), Phospho (ST), Phospho (Y); (f) mass tolerance for precursor ions: ±100 ppm; (g) mass tolerance for fragment ions: ±0.2 Da; (h) name of database searched and release version: NCBInr 20080628 (6655203 sequences); (i) species restriction: Mammalia (mammals) (689751 sequences); (j) acceptance criteria: cut-off score value for protein: 71 (score is -10Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant ()).
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