Leptin and Fasting Regulate Rat Gastric Glucose-Regulated Protein 58

Table 3

Identification of GRp58 protein in the gastric mucosa of the rat. Rat gastric proteins were separated by 2-DE and identified by means of MALDI-TOF. The protein identified represents the spots shown in Figures 1(a) and 1(b): protein disulfide-isomerase A3 precursor (Disulfide isomerase ER-60; ERp60; 58 kDa microsomal protein; p58; ERp57; HIP-70; Q-2; 35 petides), the spots labelled as P1′ and P2′ are the same spots as P1 and P2 but in different gels.

Protein MW: 57043.9  Protein PI: 5.88  Accession No: gi|1352384|
Observed Mr (expt)	Mr (calc)	± da	± ppm	Start-end	Sequence	Spot number
823.4785	823.4559	0.0226	27	190–196	IVAYTEK	p1′,p1
866.4574	866.4617	−0.0043	5	387–393	YKELGEK	p1′,p1
877.4915	877.489275	0.0025	3	268–275	LNFAVASR	p1′,p1
995.5628	995.5632	−0.0004	0	102–111	QAGPASVPLR	p1′,p1
997.5101	997.459	−0.0511	−51	153–161	DASVVGFFR	p2
1084.5674	1084.5688	0.0014	1	95–104	YGVSGYPTLK	p2′,p1,p2,P3
1123.6582	1123.6589	0.0007	1	101–111	KQAGPASVPLR	p1′,p1
1125.547	1125.5436	0.0034	3	243–251	NTKGSNYWR	p1
1172.5404	1172.4702	−0.0702	−60	336–344	FVMQEEFSR	p1,p2
1179.5865	1179.6239	0.0374	32	174–183	AASNLRDNYR	p2′,p1,p2
1188.5354	1188.5305	−0.0049	−4	344–336	FVMQEEFSR	p2′,p1,p2,P3
1191.6005	1191.6084	0.0079	7	63–73	LAPEYEAAATR	p1′,p2′,p1,p2,P3,P4
1236.5127	1236.5029	−0.0098	−8	108–119	DGEEAGAYDGPR	p2′,p1,p2,P3
1244.6633	1244.6276	−0.0357	−29	184–194	FAHTNVESLVK	p1,P3
1341.6837	1341.683	−0.0007	−1	449–460	GFPTIYFSPANK	p2′,p1,p2,P3,P4
1347.7043	1347.7015	0.0028	2	33–44	RLAPEYEAAATR	p1
1373.6736	1373.5839	−0.0897	−65	352–362	FLQEYFDGNLK	p2
1394.6587	1394.5729	−0.0858	−62	162–173	DLFSDGHSEFLK	p1,p2
1396.6954	1396.6727	−0.0227	−16	367–379	SEPIPETNEGPVK	p2′,p1,p2,P3,P4
1397.5784	1397.7063	0.1279	92	83–94	VDCTANTNTCNK	p1′,p2′,p2,P3,P4
1397.7059	1397.7063	0.0004	0	472–482	ELNDFISYLQR	p1′,p2′,p1,P3,P4
1469.7787	1469.691	−0.0877	−60	449–461	GFPTIYFSPANKK	p1,p2,P4
1472.6838	1472.6184	−0.0654	−44	336–347	FVMQEEFSRDGK	p2
1488.6787	1488.6698	−0.0089	−6	336–347	FVMQEEFSRDGK	P3
1529.7747	1529.775	0.0003	0	352–363	FLQEYFDGNLKR	p1′,p2′,p1,p2,P3,P4
1593.8483	1593.8551	0.0068	4	483–496	EATNPPIIQEEKPK	p2′,p1,p2,P3,P4
1607.7476	1607.764	0.0164	10	259–271	DLLTAYYDVDYEK	p1′,p2′,p2,P3,P4
1636.7523	1636.6552	−0.0971	−59	434–448	MDATANDVPSPYEVK	p1,p2,P4
1652.7472	1652.6615	−0.0857	−52	434–448	MDATANDVPSPYEVK	p2
1652.7662	1652.7704	0.0042	3	105–119	IFRDGEEAGAYDGPR	p1′,p2′,p1,p2,P3,P4
1744.8864	1744.7698	−0.1166	−67	131–146	QAGPASVPLRTEDEFK	p2
1746.9286	1746.8311	−0.0975	−56	289–304	TFLDAGHKLNFAVASR	p1′
1800.9377	1800.9132	−0.0245	−14	364–379	YLKSEPIPETNEGPVK	p1′,p2′,p1,p2,P3,P4
1950.9331	1950.7816	−0.1515	−78	259–274	DLLTAYYDVDYEKNTK	p2
2463.1279	2462.9673	−0.1606	−65	83–104	VDCTANTNTCNKYGVSGYPTLK	p2