Intracellular Loop 2 Peptides of the Human 5HT1a Receptor are Differential Activators of Gi

Figure 3

ic2 peptide effect on forskolin-stimulated cAMP production, a measure of activated-G-protein regulation of adenylyl cyclase. Forskolin (FSK) stimulated cAMP production by adenylyl cyclase (AC) is in CHO cells expressing the human 5HT1aR. FSK (30 uM) is the control, which is set to 100%. All other treatments are expressed as a percent of the control value. Peptide concentrations are 30 uM. All treatments include isobutylmethylxanthine (IBMX) an inhibitor of the metabolism of cAMP by phosphodiesterase. Error is expressed as SEM.