Intracellular Loop 2 Peptides of the Human 5HT1a Receptor are Differential Activators of Gi
Figure 5
P12 and P13 effect of forskolin-stimulated cAMP production forskolin (FSK) stimulated cAMP production by adenylyl cyclase (AC) in CHO cells expressing the human 5HT1aR. These experiments were a measure of second messenger regulation by G protein. FSK is the control, which is set to 100%. All other treatments are expressed as a percent of the control value. Peptide concentrations are 30 uM. All treatments include isobutylmethyl xanthine (IBMX), an inhibitor of the metabolism of cAMP. 5HT versus 5HT/P12 and 5HT versus 5HT/P13 *.
