Peptidomic Analysis of the Brain and Corpora Cardiaca-Corpora Allata Complex in the Bombyx mori

Table 2

The mature peptides of Bombyx mori from larvae CC-CA identified or assigned by MALDI-TOF MS.

Number	Peptide clusters	Maturae peptide name	Amino acid sequence	Reference	Transcripts	Monoisotopic masses M+H]^+		Larvae CC-CA
						Calculated	Measured
1	AKH	AKH1(Q^1)	QLTFTSSWG-NH2	[3, 13]	AB298930	1025.48	1025.58	+
		AKH2(Q^1)	QLTFTPGWGQ-NH2	[13]	AB298931	1133.55	1133.78	+
		AKH3(Q^1)	QITFSRDWSG-NH2	[13]	AB298938	1195.56	1194.85	+
2	Allatostatin A	Allatostatin-2	AYSYVSEYKRLPVYNFGL -NH2	[9]	AF309090	2168.09	2167.621	+
		Allatostatin-3	SRPYLFGL-NH2	[9]		951.52	951.41	+
		Allatostatin-4	ARPYSFGL-NH2	[9]		909.47	909.32	+
		Allatostatin-5	ARMYSFGL-NH2	[9]		943.46	943.46	+
		Allatostatin-6	ARSYSFGL-NH2	[9]		899.45	899.52	+
		Allatostatin-8(Q^1)	QRDMHRFSFGL-NH2	[9]		1392.67	1393.88	+
		Allatostatin-8	pERDMHRFSFGL-NH2	[9]		1375.7	1375.60	+
3	Bommo-AT	Bommo-AT	GFKNVEMMTARGF-NH2	[4]	AY970687	1486.71	1486.69	+
4	Bommo AST-C	AST-C(Q^1)	QVRFRQCYFNPISCF-NH2	[12]	AB330418	1906.90	1960.96	+
5	BRFa	BRFa-1	SAIDRSMIRF-NH2	[5]	AB234100	1194.62	1194.85	+
		BRFa-2	SASFVRF-NH2	[5]		812.42	812.42	+
		BRFa-3	DPSFIRF-NH2	[5]		880.44	880.48	+
		BRFa-4	ARNHFIRL-NH2	[5]		1025.59	1025.63	+
6	B-myosuppressin (BMS)	BMS	pEDVVHSFLRF-NH2	[6]	NM_001173411	1229.6	1229.54	+
		BMS(Q^1)	QDVVHSFLRF-NH2	Confirmed in this study		1246.65	1246.55	+
7	CAPA/CAP2b	CAPA-PVK-1^⋇	PDGVLNLYPFPRV-NH2		AB362227	1485.80	1486.54	+
		CAPA-PVK-2^⋇	pELYAFPRV-NH2			975.50	975.71	+
		CAPA-PVK-2(Q^1)^⋇	QLYAFPRV-NH2			992.54	992.80	+
8	CCAP	CCAP	PFCNAFTGC-NH2		AB298937	958.37	956.42	+
9	Corazonin	Corazonin	pETFQYSRGWTN-NH2	Confirmed in this study	AB106876	1369.7	1369.57	+
		Corazonin(Q^1)	QTFQYSRGWTN-NH2	Confirmed in this study		1386.63	1385.74	+
10	NPLP-1	NPLP-1(LLH)^⋇	NIAALARNGLLH-NH2		AB362222	1261.73	1261.57	+
		NPLP-1(NSY)^⋇	NIATLAKNGYLRNSGANSY-NH2			2026.02	2027.19	+
11	Orcokinin	Orcokinin-5	YRPDYPMDEIDLSHFPVGS-NH2	Confirmed in this study	AB298932	2237.01	2237.39	+
12	PTST	PTST-3	APEKWAAFHGSWG-NH2	[15]	AB073553	1442.67	1441.83	+
		PTST-5	AWQDMSSAW-NH2	[15]		1080.43	1080.67	+
		PTST-6	AWSALHGTW-NH2	[15]		1027.49	1027.67	+
13	SGNP	α-SGNP	IIFTPKL-NH2	[4]	D28810	830.53	830.78	+
		β-SGNP	SVAKPQTHESLEFIPRL-NH2	[4]		1951.05	1951.32	+
		γ-SGNP	TMSFSPRL-NH2	[4]		937.47	937.67	+
14	sNPF	sNPF-1	SPSRRLRF-NH2	[10]	AB330419	1017.58	1017.45	+
		sNPF-2	TPVRLRF-NH2	[10]		887.53	887.72	+
		sNPF3	APSMRLRF-NH2	[10]		976.53	976.74	+
15	Sulfakinin	Sulfakinin^⋇	GDDTFDDYGHLRF-NH2		AB362223	1556.65	1556.92	+
16	Tachykinin	Tachykinin-1^⋇	IPQGFLGMR-NH2		AB298929	1017.54	1017.60	+
		Tachykinin-3^⋇	APLGFTGVR-NH2			916.51	916.50	+
		Tachykinin-4^⋇	AANMHQFYGVR-NH2			1292.61	1292.48	+
		Tachykinin-6^⋇	GQMGFFGMR-NH2			1029.45	1029.74	+

Masses were calculated using ProteinProspector (University of California, San Francisco, CA, USA). The mature peptide marked. ^⋇Newly characterized peptides in this study.