Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea
Figure 4
Sequence alignment of GTx-TCTP (a) and GTx-CRISP (b) families. The putative signal sequence deduced by SignalP 3.0 server (http://www.cbs.dtu.dk/services/SignalP/) is indicated by dotted box. Conserved cysteine residues are indicated by closed boxes filled with gray color. (c) The results of real-time PCR. G3PDH is equally expressed in the pereopodal muscle (PM) and the venom gland (VG). GTx-CRISP transcript is predominantly expressed in the venom gland. The GTx-CRISP transcript in the PM is one-400th of that in the VG. GTx-TCTP transcript is expressed in both the pereopodal muscle and the venom gland. The GTx-TCTP transcript in the PM is one-13th of that in the VG. Results were confirmed in triplicate experiments. Accession numbers: GTx-TCTP, AB201040; HumanTCTP, NM_003295; Bombyx mori (Bm) TCTP, NM_001044107; Dermacentor andersoni (Da) HRF, DQ009480; GTx-CRISP, AB201041; Pseudecin (Harpegnathos saltator), EFN80524; Human-CRISP1, NM_001205220; Helothermine, U13619; Ablomin, AF384218.
