Effect of Rabbit Epididymal Antimicrobial Peptide, REHbP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro
Figure 8
Determination of U937 cell migration/infiltration using chemotaxis assay. Culture supernatants of hVECs were collected after the cells were stimulated with LPS (10 g for 1 h). When these supernatants were placed in the lower Transwell chamber, U937 cell migration from upper Transwell was significantly increased over that of medium control. When supernatants obtained from LPS-induced cells that were treated with REHbP (60.61 or 15.50 M for 1 h) and used in the lower Transwell chamber, the migration of U937 cells was reduced in a dose-dependent manner. REHbP at a concentration of 60.61 M resulted higher inhibition of cell migration. As expected scrambled peptide did not suppress LPS-induced infiltration of U937 cell. The data was normalized to U937 chemotaxis in response to 0.1 mM fMLP in PBS/BSA, which was used to stimulate cell migration (100%). Each value is the mean ± S.D. of six individual observations obtained from three independent experiments. Levels of significance ( compared with the LPS-induced group, compared with the LPS-induced group, compared with the medium control) were calculated by ANOVA test followed by a Bonferroni analysis.
