Nociceptin Signaling Involves a Calcium-Based Depolarization in Tetrahymena thermophila

Table 2

Pharmacological inhibitors which act on G-protein mediated receptor pathways and tyrosine kinase pathways do not significantly impact nociception avoidance. 𝑁 represents the number of trials conducted. Each trial consisted of 10 cells, which were individually scored as positive or negative for avoidance.

Pharmacological inhibitor	Pathway inhibited	Percentage of cells avoiding nociceptin	𝑁
Control	None	94.28 ± 5.34	8
50 μM RpcAMPs	Adenylyl cyclase	96.67 ± 5.16	6
1 mM GDP-𝛽-S	G-proteins	100.0 ± 0.0	6
1 μM U-73122	Phospholipase C	97.78 ± 4.40	9
1 μM U-73345	Inactive analogue of U-73122	96.67 ± 5.16	6
10 μM calphostin C	Protein kinase C	96.0 ± 5.0	10
1 mM 1400 W	NOS	96.0 ± 5.0	10
100 μM tyrphostin 47	Receptor tyrosine kinases	92.5 ± 9.57	6
100 μM AG126	Map kinase pathway	96.0 ± 6.99	10
150 μM SU 6668	Receptor tyrosine kinases	91.66 ± 4.08	6
300 μM apigenin	Protein kinases	91.2 ± 6.4	8
3 mM H-9	Protein kinases	96.6 ± 5.1	6