High Proteolytic Resistance of Spider-Derived Inhibitor Cystine Knots

Figure 2

ProTx-II stability in rat plasma. ProTx-II degradation in rat plasma was accessed in vitro and in vivo. Results are mean ± SEM for 3 experiments. (a) ProTx-II degradation in rat plasma in vitro. No ProTx-II degradation was observed in rat plasma in vitro for 24 hours. (b) ProTx-II concentration in rat circulation blood in vivo. ProTx-II was rapidly eliminated from the circulation. Plasma half-life was 40 min and ProTx-II concentration dropped below the detection limit in 8 hours.

(a)

(b)